| Physical and Biological Activity of Domestic Product of Modified Bovine Lung Surfactant |
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Chul Lee1, Min Soo Park1, Moon Sung Park1, Jung Nyeon Kim1, Jong Wook Lee2, Kwang Hyun You2, Won Jae Kwag2, Kook In Park1, Ran Nam gung1, Dong Gwan Han1 |
1Department of Pediatrics, Yonsei University College of Medicine
2Yuhan Research Center |
| 국내 개발된 Modified Bovine Lung Surfactant의 물리학적 및 생물학적 활성도 |
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이철1, 박민수1, 박문성1, 김정녕1, 이종욱2, 유광현2, 곽원재2, 박국인1, 남궁란1, 한동관1 |
1연세대학교 의과대학 소아과학교실
2유한양행 중앙연구소 |
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| Abstract |
Purpose
: Neonatal respiratory distress syndrome is caused by the deficiency of lung surfactant in
premature babies. For the treatment of RDS at present surfactants such as Surfacten․
(Tokyo-Tanabe Co., Japan) and Exosurf․ (Wellcome Co., USA) are used. As awarded the grant
from the Ministry of Science and Technology for a model research project of Medium-Technology
program, we have modified (supplemented) the bovine lung extracts to get YY-38, for which we
have performed physical and biological activities.
Methods
: For physical properties, we performed stable microbubble test (SMR) and measured
surface tension lowering activity using a pulsating bubble surfactometer. Minimum and maximum
surface tensions measured at 1 and 5 minutes gave surface tension-surface area diagrams, from
which compressibility at surface tension 10mN/m was also calculated. As to the biological activity,
we used premature rabbit fetuses as a model for the study of pressure-lung volume relationship.
The lung pathology was examined on the lung tissues subsequently obtained, and aerated area
ratios were calculated based on the area measured by an image analyzer.
Results
: The minimum surface tensions of YY-38 at 1 and 5 minutes for all different concentrations
were low at 10mN/m, while the maximum surface tensions ranged from 33.01mN/m to
41.07mN/m. The surface tension-surface area curve showed a definite hysteresis at 1 and 5
minutes for all concentrations, and the surface tension fell below 10mN/m with 20% surface area
compression. The compressibilities at surface tension 10mN/m at 5minutes for all concentrations
were all below 0.02. In animal experiments, the mean lung volume of premature rabbit fetuses was
inflated to 80.9ml/kg at maximum 30cmH2O, while the lung volume was maintained at 38.3mg/kg
when the lung was deflated to 5cmH2O. The overall aerated area ratio was 45.4%.
Conclusion
: YY-38 formed sufficient amount of stable microbubbles and had a surface tension
low enough to maintain alveolar stability and to exhibit a good hysteresis curve. In animal
experiments it helped the expansion of premature lungs during inspiratory phase and was effective
in the prevention of collapse during expiratory phase. |
| Key Words:
Modified bovine lung surfactant, Fetal rabbit lung, Pressure-lung volume curve, Surface tension-surface area diagram |
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