| Peripheral-Blood-Based PCR Assay to Identify Patients with Childhood Tuberculosis |
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Soo Sung Park1, Byoung Moon Ahn1, Eun Ryoung Kim1, Il Soo Kim1, Jae Jong Kim2, Yu Jin Rha2, Han Ho Park2 |
1Department of Pediatrics, Sung Ae General Hospital, Seoul, Korea
2Bioneer Corp. R& D Center |
| 말초 혈액을 이용하여 Nested PCR법에 의한 소아 결핵의 진단 |
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박수성1, 안병문1, 김은령1, 김일수1, 김재종2, 나유진2, 박한오2 |
1성애병원 소아과
2바이오니아부설연구소 |
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| Abstract |
Purpose
: There is an urgent need for rapid and accurate diagnosis of childhood tuberculosis.
Recently, developments in molecular biology have raised hopes about the possibilities of new
strategies for tuberculosis diagnosis. Most of these methods have focused on the application of
PCR to sputum samples from patients with suspected mycobacterial disease. We used a nested
PCR to detect circuclating Mycobacterial tuberculosis DNA in blood samples from patients with
suspected tuberculosis infection. We have propspectively investigated the role of blood-based PCR
assay for diagnosis of childhood tuberculosis.
Methods
: Our PCR assay is specific for IS6110 insertion element of the M.tuberculosis complex
of organisms. We used it to test peripheral blood from 179 children with suspected tuberculosis
infection. Results of the PCR assay were compared with tuberculin test and contact history with
pulmonary tubercosis patients. A subgroup of 16 patients has blood samples assayed serially to
track the PCR signal after treatment.
Results
: In patients with contact history, 20 out of 30 samples(66.7%) were PCR positive. In
patients with tuberculin test positive, 43 out of 78 samples(61.59%) were PCR positive. However
there was no significant difference between weak(10-15mm) and strong(> 15mm) tuberculin
responsor groups. Negative conversion of PCR signal was observed in 14.3% (1/7) of subjects
after 2 months of treatment, and 71.4% (5/7) after 3 months of treatment. Positive results of PCR
was not observed after BCG vaccination.
Conclusion
: We conclude that peripheral-blood-based PCR detection for diagnosis of childhood
tuberculosis is technically feasible approach that has a potential important role in diagnosis of
childhood tuberculosis. |
| Key Words:
Mycobacterial tuberculosis, Blood-based PCR, Tuberculin test |
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