| Rapid Identification of Mycoplasma Pneumoniae, Mycobacterium
Tuberculosis and Staphylococcus Aureus in Pleural Fluid by PCR |
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Chang Wan Kim1, Byung Moon Ahn1, Eun Ryoung Kim1, Il Su Kim2, Yung Seuk Pak3, Sang Chul Sung3 |
1Department of Pediatrics, Sung-Ae General Hospital, Seoul, Korea
2Sung-Ae Genentic Engeenearing Research Institute
3Department of Molecular Biology, Seoul Medical Science Institute, Seoul, Korea |
| 감염성 늑막염의 늑막삼출액으로부터 중합효소연쇄반응을 이용한 마이코플라즈마균, 결핵균 및 황색 포도상구균의 특이 유전자분석의 임상적 의의 |
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김창완1, 안병문1, 김은령1, 김일수2, 박영석3, 성상철3 |
1성애병원 소아과
2성애 유전공학 연구소
3서울의과학 연구소 |
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| Abstract |
Purpose
: Pleural effusions may develop during the course of bacterial pneumonia. The
aim of this study was to evaluate the significance of the polymerase chain reaction(PCR)
method
for detection of M ycoplasma pneumoniae, M ycobaterium tuberculosis and
Staphylococcus aureus from pleural fluid.
Methods
: Total 12 samples were obtained from pleural fluid; 2 samples from children
with Mycoplasma pneumonia, 5 samples from adults with tuberculous pleurisy, and 5
samples from sterile pleural fluid seeded artificially with staphylococcus aureus. The
primers used for our PCR were prepared to amplify M . pneumonia-specific MP5 gene,
M. tuberculosis-specific IS6110 gene, and S. aurus-specific femA and mecA gene. The
amplified PCR products were detected by ethidium bromide-stained agarose gel electrophoresis.
Results
: A total of 12 pleural fluid samples were tested by nested PCR using the
specific primer set. We could amplify MP5 gene in 2 samples, IS6110 gene in 5 samples,
mecA gene in 3 samples, and femA gene in 5 samples. These PCR data were correlated
with serolological data, microbiological data and methicillin-sensitivity test result. There
were no false-positive results due to cross-contaminating DNA between these 3 organisms.
Conclusions
: We conclude that enzymatic amplification of specific gene from pleural
fluid might be useful to diagnose the infectious pleural effusion by M ycoplasma pneumoniae,
M ycobacterum tuberculosis or Staphylococcus aureus. |
| Key Words:
M ycoplasma pneumoniae, M ycobacterium tuberculosis, Staphylococcus aureus, Pleural effusion, Polymerase chain reaction |
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