| Basophil Histamine Releasability in Asthmatic Children |
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Seon Mi Jin, Jin Hwa Jung, Yong Han Sun, Myung Hyun Lee, Joong Gon Kim, Young Yull Koh |
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Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea |
| 천식 환아의 호염기구 히스타민 유리능에 관한 연구 |
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진선미, 정진화, 선용한, 이명현, 김중곤, 고영률 |
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서울대학교 의과대학 소아과학교실 |
Correspondence:
Young Yull Koh, Email: 1
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| Abstract |
Purpose
: The release of histamine from human basophils is controlled by an intrinsic, as yet
unidentified, cellular property termed “releasability.” We carried out this study to ascertain whether
there was any difference in the releasability of basophils from asthmatic children compared to
those from normal children. We intended also to clarify the correlation between the releasability
and the atopic status of asthma.
Methods
: We selected nineteen atopic asthmatic, eighteen nonatopic asthmatic and fourteen
normal children for this study. Suspensions of leukocytes were isolated and stimulated with
calcium ionophore A23187, anti-IgE and D. pteronyssinus antigen. After incubation, the supernatant
was assayed for histamine with an automated fluorometric technique.
Results
: Basophil histamine release with anti-IgE was different in three groups. Anti-IgE caused
significantly more basophil histamine release in asthmatic children than in nomal children. Atopic
asthmatic group showed greater basophil histamine release with anti-IgE than nonatopic asthmatic
group. D. pteronyssinus antigen caused the significant amount of histamine release only in atopic
asthmatic group.
Conclusions
: Our data suggests that basophils from asthmatic children are characterized by a
specific increase in IgE mediated histamine releasability. The difference of histamine releasability
with anti-IgE between atopic and nonatopic asthmatic children may be due to the heterogeneity of
IgE bound to cell surface, or may be due to the degree of the basophil activation by cytokines
such as IL3. The specific release of histamine with D. pteronyssinus antigen in atopic asthmatic
group suggests that the basophil histamine release test can be used to diagnose the causing
antigen. |
| Key Words:
Basophil histamine release, Atopic asthma, Nonatopic asthma |
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