| Congenital Asymptomatic Cytomegalovirus Infection; A Comparison of Specific IgM Antibody Test and pp65 Antigenemia Assay |
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Jong Hyun Kim1, Eun Ah Seo1, Ji Hyang Lim2, Sang Hee Park3, Won Bae Lee1, Kyong Su Lee1, Sin Ho Jung4 |
1Department of Pediatrics, Catholic University College of Medicine, Seoul, Korea
2Department of Clinical Pathology, Catholic University College of Medicine, Seoul, Korea
3Medical Research Institute of St. Holy Hospital, Pucheon, Korea
4Department of Statistics, Hallym University, Chuncheon, Korea |
| 선천성 무증상 거대세포 바이러스 감염; 특이 IgM 항체검사법과 pp65 항원혈증법의 비교 |
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김종현1, 서은아1, 임지향2, 박상희3, 이원배1, 이경수1, 정신호4 |
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 임상병리학교실
3성가병원 임상의학연구실
4한림대학교 통계학과교실 |
Correspondence:
Jong Hyun Kim, Email: 1
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| Abstract |
Purpose
: It is increasingly important to diagnosis asymptomatic infections which make up a majority(90%) of congenital cytomegalovirus(CMV) infections and that they may have sequeles such as sensorineural hearing loss and mental retardation. Recently antigenemia assay has been developed by using monoclonal antibodies against early structural protein pp65 of CMV. This CMV antigenemia assay seems to be more quicker to diagnosis than conventional viral culture or other tests. In this study, we evaluated the CMV antigenemia assay in neonatal congenital asymptomatic CMV infections comparing it to the CMV specific IgM test that uses enzyme immunoassay.
Methods
: From October 1995 to May 1996, 231 normal term newborns delivered with asymptomatic in St. Holy Hospital of Catholic University were included. The CMV antigenemia assay was performed with CMV-vueTM Kit by immunocytochemical staining and the CMV specific IgM test was performed with EnzygnostⓇ Anti-CMV/IgM by using an enzyme immunoassay.
Results
: Three cases(male 2, female 1) were CMV pp65 antigenemia assay positive, but none of them were CMV specific IgM antibody test positive. The CMV pp65 antigenemia assay was more sensitive than CMV specific IgM antibody test for detection of congenital asymptomatic CMV infections by 1.3% and 0%, respectively.
Conclusion
: According to previous results, we suggest that the rate of congenital CMV infections using only CMV specific IgM tests have been underestimated. We recommend the CMV antigenemia assay as the preferred method for more rapid and accurate diagnosis of CMV infections. And congenital asymptomatic CMV infections should be diagnosed and followed up because of possible future sequeles. |
| Key Words:
Cytomegalovirus, Congenital infection, pp65 antigenemia assay, IgM antibody |
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