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Effects of Cefodizime on Phagocytosis of COS-1 Ccells

Journal of the Korean Pediatric Society 1998;41(12):1632-1638.
Published online December 15, 1998.
Effects of Cefodizime on Phagocytosis of COS-1 Ccells
Pyoung Han Hwang1, Sang Yun Nam2, Ho Keun Yi3, Min Ho Kim4, Kong Soo Kim4, Jung Soo Kim3
1Institute for Medical Sciences, Chonbuk National University
2Department of Biology, College of Science and Technology, Chonju University
3Department of Pediatrics, School of Medicine, Chonbuk National University
4Department of Cardiovascular Surgery, School of M edicine, Chonbuk National University
COS-1 세포의 탐식작용에 대한 Cefodizime의 효과
황평한1, 남상윤2, 이호근3, 김민호4, 김공수4, 김정수3
1전북대학교 의과대학 의과학 연구소
2전주대학교 이공대학 생물학부
3전북대학교 의과대학 소아과학교실
4전북대학교 의과대학 흉부외과교실
Correspondence: 
Pyoung Han Hwang, Email: 1
Abstract
Purpose
: Cefodizime is a new third-generation cephalosporin which has a structure and immunomodutation properties similar to cefotaxime. Various studies on cefodizime have demonstrated the direct eradication of bacteria in cooperation with the host defense mechanism, particularly with phagocytosis. We evaluated the effects of cefodizime on the phagocytosis of COS-1 cells transfected with FcγRI/γγ or FcγRIIA cDNA.
Methods
: Phagocytosis was measured using the in vitro COS-1 cell modeling system according to Schreiber's method. COS-1 cells, which lack endogenoous Fcγreceptors but have phagocytic potential, were transfected with either FcγRI/γγor FcγRIIA cDNA. COS-1 cells, as target cells, were treated with antibiotics for 1 or 24 hours and incubated for 30 min with IgG coated sheep RBCs. Adhered IgG coated sheep RBCs were removed after brief exposure to hypotonic phosphate buffered saline. Phagocytosis index(PI) was calculated as the number of ingested RBCs per 100 phagocytic cells after wright-Giemsa staining.
Results
: COS-1 cells tranfected with FcγR(either FcγRI/γγ or FcγRIIA cDNA) showed the phagocytic activity against IgG coated sheep RBC, while untransfected COS-1 cells did not. After treatment with cefodizime, phagocytic activity of FcγRI/γγcDNA transfected COS-1 cells was significantly increased, while that of FcγRIIA cDNA transfected COS-1 cells did not. Marked enhancement of phagocytosis of COS-1 cells was observed after treatment with cefodizime, but was not observed with ceftriaxone or moxalactam.
Conclusion
: Cefodizime showed marked enhancement of phagocytic activity of FcγR transfected COS-1 cells. FcγRI seems to play an important role in the enhancement of phagocytosis. Further studies will be required.
Key Words: Cefodizime, In vitro COS-1 cell modeling system, Phagocytosis


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