| Prevalence and Identification of Rotaviruses in Stool Specimens of Patients with Acute Diarrhea from Several Regions of Korea |
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Dong Soo Kim1, Beom Soo Park2, Dong Hyuk Jung3, Jae Moon Ahn4, Chul Joong Kim5, Shien Young Kang6 |
1Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea
2Department of Pediatrics, College of Medicine, Chungbuk National University, Cheongju, Korea
3Green Cross Co. Ltd., Seoul, Korea
4Institute of Livestock and Veterinary Services
5College of Veterinary Medicine, Chungnam National University∫, Taejeon, Korea
6College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea |
| 국내 일부지역 설사환자의 로타바이러스 감염실태 및 분리동정 |
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김동수1, 박범수2, 정동혁3, 안재문4, 김철중5, 강신영6 |
1연세대학교 의과대학 소아과학교실
2충북대학교 의과대학 소아과학교실
3녹십자
4축산위생 연구소
5충남대학교 수의과대학
6충북대학교 수의과대학 |
Correspondence:
Dong Soo Kim, Email: 1
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| Abstract |
Purpose
s : Rotaviruses are the main cause of infantile diarrheal diseases worldwide. The purpose of this study is to obtain epidemiologic data of rotavirus infections in Korea.
Methods
: Stool specimens were collected from 150 patients with acute diarrheal symptoms, who were admitted to Yonsei Medical Center and Chungbuk National University Hospital. After isolating the virus from the specimens, the viruses were identified as rotaviruses by electron microscope and fluorescent microscope after staining with rotavirus VP6-specific monoclonal antibody. RNA was extracted from the specimens by modified phenol/chloroform method. Electropherotying was done with extracted RNA samples after silver staining of the gels. Rotavirus serotyping was done using commercial serotyping ELISA kit.
Results
: Rate of infection from rotavirus stool samples was 60%. Isolated rotaviruses were all serogroup A and a majority(46.0%) of these viruses were long type. Serotypes 1 and 2 were identified with serotype 1 being the majority(52.2%). There were no differences in these types between 1991 and 1992, and also between Seoul and Cheongju area.
Conclusion
: Electropherotype of rotavirus epidemic in Korea was serogroup A, and long type in the majority. Serotypes of rotavirus epidemic in Korea were type 1 and type 2. These results show that the rotavirus reassortant vaccine developed in America could also handle the rotaviral disease in Korea. |
| Key Words:
Rota virus, Electropherotype, Serotype |
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