| Immunohistochemical Expression of c-fos Protein and Histologic Findings after Instillation of Kainic Acid in Hippocampus of Neonatal Rat Brain |
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Soo Ahn Chae, Yong Soo Kim, Byoung Hoon Yoo, Won Bok Lee |
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Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, Korea |
| 신생 쥐 해마부위의 Kainic Acid 투여로 인한 c-fos 단백 발현 양상 및 조직학적 소견에 관한 연구 |
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채수안, 김용수, 유병훈, 이원복 |
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중앙대학교 의과대학 소아과학교실 |
Correspondence:
Soo Ahn Chae, Email: 1
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| Abstract |
Purpose
: c-fos is rapidly and transiently induced in the intact CNS by a wide variety of exogenous stimuli that include seizures, glutamate receptor activation, sensory stimulation and stress. In adult animals, systemic KA administration produces limbic seizures that results in c-fos protein expression, irreversible morphological changes and localized neuronal death. So we studied the pattern of c-fos protein expression and histological findings in hippocampal formation, following kainic acid-induced seizures during the postnatal period in the rat.
Methods
: Sprague-Dawley rat pups ranging from 8 to 10 days of age, received kainic acid(KA, 5mg/kg) by intraperitoneal injection. Control rats were injected with normal saline. The rats were perfused and fixed with 4% buffered paraformaldehyde at varying time-intervals after KA injection, the brains were sectioned and immunohistochemically stained for c-fos protein and performed HE staining.
Results
: In the hippocampus, immunohistochemistry showed that c-fos protein expressed at 12 hr after KA injection and disappeared thereafter. c-fos protein expressed in all sectors of the hippocampus but most densely expressed in CA1 and CA3 sectors. Rarely c-fos expression was seen in the granular cell layer of dentate gyrus. There were no histologic changes in the hippocampus at 2 weeks after KA injection.
Conclusion
: c-fos, a proapoptotic gene in adult rats, seemed to have an additive role in neuronal cell adaptation to exogenous stimuli in neonatal rats. As a result, it suggests that the roles of c-fos in neuronal cells after noxious stimuli are different between neonatal and adult rats. |
| Key Words:
Kainic acid, c-fos, Neonatal rat brain |
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