| Comparison Study between Immunohistochemical Staining and Enzyme Linked Immunosorbent Assay in Metalloproteinases and Tissue inhibitor of Metalloproteinases in Virus induced Myocarditis |
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Young Mi Hong1, Seung Sook Kim2 |
1Department of Pediatrics, Ewha Womans University, College of Medicine, Seoul, Korea
2Department of Pathology, Ulsan University, College of Medicine, Seoul, Korea |
| 바이러스로 유도된 심근염에서 Metalloproteinase와 Metalloproteinase 조직 억제자의 면역조직화학염색에 대한 발현도와 효소면역법과의 비교 연구 |
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홍영미1, 김성숙2 |
1이화대학교 의과대학 소아과학교실
2울산대학교 의과대학 해부병리학교실 |
Correspondence:
Young Mi Hong, Email: 1
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| Abstract |
Purpose
: Most tissue disruption of extracellular matrix is mediated by extracellular proteinases. Matrix metalloproteinases(MMP) and tissue inhibitor of matrix metalloproteinases(TIMP) are associated with wound healing and repair. There has been no study done on MMP and TIMP in myocarditis.
Methods
: Coxsackie B virus(4,000 plaque forming unit) was injected into Balb/c mice by intraperitoneal injection. Histopathological finding was observed by H-E staining. Immunohistochemical staining was performed using the Labelled Streptavidin Biotin(LSAB) kit for MMP-2, TIMP-2, and Interleukin(IL) 6. The results were compared to the serum levels by ELISA method.
Results
: MMP-2 was strongly expressed in complicated myocarditis such as calcification, severe fibrosis, thrombosis, or dilated cardiomyopathy compared to normal or uncomplicated myocarditis. TIMP-2 expression was decreased in severe myocarditis. Serum MMP-2 levels were significantly higher in complicated myocarditis, but TIMP-2 levels were significantly lower. There was significant correlation between the grade of immunohistochemical staining and serum MMP-2 or TIMP-2 level by ELISA method. IL-6 was strongly expressed in immunohistochemical staining according to the severity of inflammation.
Conclusion
: There was significant correlation between grade of immunohistochemical staining and serum levels of MMP or TIMP by ELISA method. Accurate estimations of serum MMP and TIMP levels would be useful for the diagnosis and follow up of myocarditis. |
| Key Words:
Coxsackie B virus, Myocarditis, MMP, TIMP |
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