| A Catalogue of Gene Expression Difference in Biliary Cirrhosis due to Biliary Atresia Using Differential Expressed Sequence Tags(EST) Screening |
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Byung-Ho Choe, Hyun-Mi Lee, Moon-Kyu Kim, Jung-Chul Kim |
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Department of Pediatrics, Liver Research Institute, Kyungpook National University School of M edicine, Taegu, Korea |
| 담도 폐쇄증 환아의 간경변 조직에서 유전자 발현의 변동 |
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최병호, 이현미, 김문규, 김정철 |
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경북대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: Extrahepatic biliary atresia is the most common indication for liver transplantation in children,
but the etiology of this disorders remains unknown. It would be very signficant to identify genes
that are specifically expressed in pathologic liver tissue of biliary atresia and analyze the pattern of
expression in those genes.
Methods
: We made dot blot panels consisting of 1,730 different EST(expressed sequence tags) clones
which were isolated from human hair dermal papilla cell cDNA library. Liver tissues were taken from
a recipient with biliary atresia and a normal donor during living-related liver transplantation. Total
RNA was extracted from each sample and reversely transcribed to make cDNA. Then radiolabelled
cDNA probe pools were made by random primed DNA labeling method and used for screening
differentially expressed genes using EST dot blot panel.
Results
: Among the total of 1,730 EST clones, 26 cDNA clones were overexpressed in biliary
cirrhosis. They revealed homology to genes encoding bcl-w, laminin binding protein, hepatocyte growth
factor-regulated tyrosine kinase substrate(HRS), thymosin β-4, 10; transforming growth factor(TGF)-β,
tissue inhibitor of metalloproteinase(TIMP)-1, signal recognition particle(SRP)4, eukaryotic initiation
factor(eIF)-2α kinase, lysyl oxidase, aldolase A, γ-glutamylcystein synthetase, collagen type I α1, 2,
collagen type Ⅲ, fibronectin, osteonectin, insulin-like growth factor binding protein(IGFBP)-2, 3, and
more. In addition, the expression of 2 clones showed that gastrula zinc finger protein and one novel
gene were decreased in biliary atresia.
Conclusion
: This study identified differentially expressed genes in biliary cirrhosis from progressive
biliary atresia using differential EST screening technique. |
| Key Words:
Gene, Biliary atresia, Hepatic fibrosis, Differential EST screening, Reverse dot hybridization |
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