| The Apoptosis and Expression of Bcl-2, Bcl-xS, Bax Proteins in Fetal Brain after Treating Pregnant Mice with Endotoxin |
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Sung Min Cho1, Seung Sook Kim2, Young Seung Hwang3 |
1Department of P ediatrics, College of M edicine, Dongguk University, Kyongju, Korea
2Department of P athology, College of M edicine, Ulsan University, Ulsan, Korea
3Department of P ediatrics, College of M edicine, Seoul National University, Seoul, Korea |
| 내독소로 유도된 임신 생쥐의 태반감염시 태아대뇌에서 관찰되는 Apoptosis와 Bcl-2, Bcl-xS, Bax 발현과의 관계 |
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조성민1, 김성숙2, 황용승3 |
1동국대학교 의과대학 소아과학교실
2울산대학교 의과대학 병리학교실
3서울대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: Apoptosis is active cell death which plays an important role in developing normal tissues.
Various conditions such as genetic defects, drugs, ischemia or infections are known to induce
apoptosis. We studied the effect of maternal infection on fetal brain development during pregnancy.
Methods
: We treated 46 C3H pregnant mice with lipopolysaccharide(LPS) or phosphat-buffered
saline and observed the changes in apoptosis and expression of bcl-2, bcl-xS, bax. The fetal brain
tissues were removed 1-48 hours after LPS treatment. The number of apoptosis per 100 neurons
and glial cells was counted in H&E stained tissue and was analyzed statistically. Immunohistochemical
staining with primary antibodies of bcl-2, bcl-xS, bax was done and their expression
was classified by the degree of staining.
Results
: The number of apoptosis was increased significantly in both neurons and glial cells of
LPS-treated group and its degree of staining was more remarkable in glial cells. Immunohistochemistry
for bcl-2, bcl-xS, bax oncoprotein revealed mildly decreased expression of bcl-2 and
markedly increased expression of bax in both neurons and glial cells, but it was more remarkable
in glial cells. Immunochemistry for bcl-xS revealed no expression in neurons and minimal expression
of bcl-xS in glial cells in both study groups.
Conclusion
: We observed an increase in the number of apoptosis, mildly decreased expression
of bcl-2 and markedly increased expression of bax in both neurons and glial cells of fetal brain
after treating pregnant mice with LPS. Maternal infection during pregnancy may have profound
effects on developing fetal brain. |
| Key Words:
Apoptosis, Lipopolysaccharide(LPS), Fetal brain, Bcl-2, Bax, Bcl-xS, Glial cell |
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