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Identification of a Calcium-activated Potassium Channel Gene Expressed in Rat Cardiac Myocytes

Journal of the Korean Pediatric Society 2000;43(7):905-912.
Published online July 15, 2000.
Identification of a Calcium-activated Potassium Channel Gene Expressed in Rat Cardiac Myocytes
In Seung Park1, Hyun-Kyu Jeon2, Nam Su Kim3, Youn Mo Ahn3, Chul-Seung Park2, Ha Baik 3
1Department of P ediatrics, Sejong General Hospital
2Department of Life Science, Kwangju
2Department of Life Science, Kwangju
3Department of P ediatrics, Hanyang University, Seoul
3Department of P ediatrics, Hanyang University, Seoul
3Department of P ediatrics, Hanyang University, Seoul
백서의 심근세포에서 발현되는 칼슘 활성화 포타슘 통로의 확인
박인승1, 전현규2, 김남수3, 안연모3, 박철승2, 이하백3
1부천세종병원 소아과
2광주과학기술원 생명과학과
2광주과학기술원 생명과학과
3한양대학교 의과대학 소아과학교실
3한양대학교 의과대학 소아과학교실
3한양대학교 의과대학 소아과학교실
Abstract
Purpose
: Calcium-activated potassium channels(KCa) may be involved in the transient outward current of the first phase of cardiac action potential. But it is still not clear whether cardiac myocytes express any KCa. We try to identify here the types of KCa expressed in rat caridac myocytes.
Methods
: We isolated total heart RNA from 50 rats(Spague-Dawley) and performed reverse transcription-polymerase chain reaction(RT-PCR) using specifically designed synthetic oligonucleotide primer sets. From the pure culture of cardiac myocyte, KCa gene expression was detected by Southern blot analysis.
Results
: RT-PCR revealed expressions of BKCa(large-conductance KCa, rSlo) and SKCa(small-conductance KCa, rSK1). We prepared cardiac myocytes pure culture(> 95% pure myocyte) using pure culture technique. RT-PCR and Southern blot analysis of rat cardiac myocyte showed only rSK1-specific band, but no rSlo-specific was detected.
Conclusion
: The expressions of more than one type of KCa are detected from rat heart. A subtype of SKCa, rSK1, was expressed in cardiac myocyte, while the main subunit of BKCa(rSlo) was found in cells other than myocytes, most likely in the smooth muscle of cardiac blood vessels.
Key Words: Heart, Cardiac Myocyte, Calcium-activated potassium channel


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