| A Study of Surface Physical Properties of New Surfactant Using Synthetic Peptides of Surfactant Protein-B |
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Chong-Woo Bae1, Yong-Mook Choi1, Joo Hyun Kang2, Sang-Chul Lee2, Kil Lyong Kim2, Kyung-Soo Hahm2 |
1Department of P ediatrics, College of M edicine, Kyunghee University, Seoul, Korea
2Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Korea |
| 표면활성제 단백 Peptides 합성을 이용한 새로운 표면활성제의 표면 물리적 특성에 관한 연구 |
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배종우1, 최용묵1, 강주현2, 이상철2, 김길룡2, 함경수2 |
1경희대학교 의과대학 소아과학교실
2생명과학연구소 단백질연구부 |
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| Abstract |
Purpose
: To produce a new generation of artificial pulmonary surfactant(PS), surfactant protein
(SP)-B from human PSwas isolated, and the amino acid sequences of these proteins were
studied. Artificial peptides of human SP-B were synthesized. New artificial PS preparations which
were composed of phospholopids and two artificial synthetic SP-B peptides were made, and the
surface physical properties of these new PS preparations were tested.
Methods
: The purities of SP-B were assessed by SDS-polyacrylamide gel and the amino acid
sequences of these proteins were determined. We synthetized two peptides SP-1 and SP-2 and
the amino acid sequences were as follows; SP-1 : RMLPQLVCRLVLRCSMD, SP-2 : RMLPQLVCRLVLRCSM.
Surface physical properties of newly artificial PSs, which were composed of a
mixture of phospholipid(PL) and SP-1 or SP-2(sample A; PL＋SP-1, sample B; PL＋SP-2), were
measured by surface spreading, adsorption rate, and surface tension-area diagram.
Results
: The amino acid sequence of human SP-B was obtained. We produced the artificial
peptides of SP-B and prepared the new generation PS(sample A and sample B). The order of the
superiority of spreading and adsorption rate was Surfacten< sample A. B< PL only. The order of that
of the absorption rate was Surfacten ≤sample A. B< PL only. We observed that newly synthesized
PS had the surface physical properties. However, these new products did not reach the in vitro
ideal level of exogenous PS.
Conclusion
: We obtained the result of surface physical properties of the new artificial PS. But
these properties did not reveal the ideal levels of artificial PS. Further studies will be needed for
the development and use of next generation exogenous PS preparations, made by synthetic SP-B
peptides. |
| Key Words:
Pulmonary surfactant, Surfactant protein(SP), SP-B, Peptides, Respiratory distress syndrome(RDS) |
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