| Tumor Vaccine Effect by IL-16 Gene Transfer into Murine Neuroblastoma Model |
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Hyun-Sang Cho, Chong Young Park |
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Department of Pediatrics, Hallym University College of Medicine, Seoul, Korea |
| 마우스 신경모세포종 모델에서 IL-16 유전자 전달에 의한 종양 백신 효과 |
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조현상, 박종영 |
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한림대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: Interleukin(IL)-16 is a potent chemoattractant factor for CD4＋ T cells, monocytes, and eosinophils. It up-regulates IL-2R on CD4＋ T lymphocytes and regulates the function of antigen presenting cells. We used retrovirus-mediated gene transfer of the human IL-16 gene into the neuro-2a cells, which is the murine neuroblastoma cell lines, to investigate whether locally secreted IL-16 might generate anti-tumor immune responses.
Methods
: We estimated whether the local secretion of IL-16 from the genetically-modified tumor cells would affect their tumorigenicity in vivo, and then, IL-16 transfected neuroblastoma cells would protect mice from tumor development after wild-type tumor cell challenges. And we investigated the mechanism of IL-16 by nude mice trial of an anti-tumor immune response.
Results
: The IL-16 gene-transduced neuro-2a clones secreted 4.2-6.0ng of IL-16 per mL per 105 cells during 24 hr. None of the mice(N=6) injected with 2×106 of irradiated, IL-16 gene-transfected neuro-2a cells developed tumors within 6 weeks while all of the mice(N=6) injected with wild-type neuro-2a cells developed tumors. Immunization of mice(N=6) with 2×106 IL-16 gene- transfected, irradiated neuro-2a cells protected these animals against a subsequent challenge with 2×106 wild-type tumor cells. Nude mice also showed an anti-tumorigenicity effect. However, the mice did not reveal the prophylactic effect against murine neuroblastoma.
Conclusion
: The local secretion of IL-16 gene-transduced tumor cells abrogated their tumorigenicity and induced protective immunity. |
| Key Words:
Interleukin-16, Neuroblastoma |
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