| The Effect of rhG-CSF and rhGM-CSF on Expression of Fcγ Receptors and β2 Integrin and Respiratory Burst Function in Human Granulocytes |
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Kyung Hyo Kim1, Kyung Ha Ryu1, Moon Kook Park2 |
1Department of Pediatrics, College of Medicine, Ewha Woman`s University, Seoul, Korea
2Department of Microbiology, College of Medicine, Ewha Woman`s University, Seoul, Korea |
| 과립구의 Fcγ 수용체와 β2 Integrin 표현 및 Respiratory Burst 기능에 대한 rhG-CSF와 rhGM-CSF의 효과 |
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김경효1, 서주영1, 박문국2 |
1이화여자대학교 의과대학 소아과학교실
2이화여자대학교 의과대학 미생물학교실 |
Correspondence:
Kyung Hyo Kim, Email: ewhakay@unitel.co.kr
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| Abstract |
Purpose
: Receptors for the Fc of IgG(FcγR) and a β2 integrin molecule, CD11c/CD18 are important in clearance of microbes by granulocytes. We performed an in vitro study on the effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF), or granulocyte-macrophage colony-stimulating factor(rhGM-CSF) on the expression of FcγR II, FcγR III, CD11c and CD18 and respiratory burst function of granulocytes.
Methods
: Peripheral blood was collected from six healthy adults. The isolated granulocytes were stimulated with rhG-CSF, 250μg/mL, rhGM-CSF, 100ng/mL or both of them for 1, 3, 9, 24, and 48 hours. Using flow cytometry, we compared the expression of the FcγR II, FcγR III, CD11c and CD18 of granulocytes before and after stimulation. We also the studied respiratory burst of granulocytes with chemiluminescence assay.
Results
: FcγR II and CD18 expression were not changed significantly after stimulation. FcγR III expression was decreased significantly after stimulation with rhG-CSF, rhGM-CSF, or both of them. CD11c expression was increased initially but was found to decrease significantly after 9 hours of stimulation. Granulocytes treated with rhG-CSF, rhGM-CSF, or both displayed enhanced respiratory burst. Our results suggest that exposure of granulocyte to growth factor results in granulocyte activation.
Conclusion
: We have shown that rhG-CSF and rhGM-CSF resulted in an activation of peripheral blood granulocytes immunophenotypically and functionally. |
| Key Words:
Granulocytes, rhG-CSF, rhGM-CSF, FcγR, β2 Integrin |
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