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Effect of Resuscitation with High Concentration Oxygen on a Rat Model of Neonatal Hypoxic-Ischemic Brain Injury

Journal of the Korean Pediatric Society 2001;44(11):1278-1288.
Published online November 15, 2001.
Effect of Resuscitation with High Concentration Oxygen on a Rat Model of Neonatal Hypoxic-Ischemic Brain Injury
Dong Seok Lee1, Su-Hee Kwak1, Heng-Mi Kim1, Yoon Kyung Sohn2
1Department of Pediatrics, College of Medicine, Dongguk University, Kyongju, Korea
1Department of Pediatrics, College of Medicine, Kyungpook University, Taegu, Korea
2Department of Pathology, College of Medicine, Kyungpook University, Taegu, Korea
신생쥐 저산소성 허혈성 뇌손상에 고농도 산소 소생술이 미치는 영향
이동석1, 곽수희1, 김행미1, 손윤경2
1동국대학교 의과대학 소아과학교실
1경북대학교 의과대학 소아과학교실
2경북대학교 의과대학 병리학교실
Correspondence: 
Heng-Mi Kim, Email: hmkim@knu.ac.kr or @knuh.knu.ac.kr
Abstract
Purpose
: This study was undertaken to determine whether any features of apoptosis would occur in the established model of cerebral hypoxia-ischemia in neonatal rats. It was also undertaken to evaluate the effect of post-insult hyperoxia on hypoxic ischemic cerebral injury.
Methods
: Seven-day-old neonatal rats underwent unilateral carotid artery dissection followed by 2 hours of hypoxia. To this end rat pups, allocated into 2 groups, were resuscitated with high concentration O2(>FiO2 95%) or room air for a 1-hour period. All of them were killed at 3 days after the above procedures. Their brains were perfusion fixed and removed to examine tissue damage by light microscope and apoptosis by terminal deoxynucleotidyl transferase mediated dUTP- biotin nick end labeling(TUNEL) reactivity.
Results
: The result demonstrates that hypoxia-ischemia model induces tissue damage and TUNEL. Post-insult exposure to high reactivity concentration O2 does not aggravate hypoxic-ischemic cerebral injury 3 days after the insult but increases TUNEL reactivity in injured tissue.
Conclusion
: These findings suggest that many cells die by apoptosis following hypoxia-ischemia in neonatal brain and resuscitation with high concentration O2 seems to provide an adverse effect over a brain injury by induction of apoptosis.
Key Words: Hypoxia, Ischemia, Newborn rat, TUNEL, Hyperoxia, Resuscitation


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