| Megakaryocyte Colony Formation of Fetal Liver Cells |
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Byung O Kwon1, Hye Young Ju1, Chun Soo Kim1, Dong Seok Jeon2, Jong In Kim3, Heung Sik Kim4 |
1Department of Pediatrics, Sungkyunkwan University, College of Medicine, Masan, Korea
2Department of Clinical Pathology, Keimyung University, College of Medicine, Taegu, Korea
3Department of Obstetrics & Gynecology, Keimyung University, College of Medicine, Taegu, Korea
4Department of Pediatrics, Keimyung University, College of Medicine, Taegu, Korea |
| 태아 간세포의 거핵구 집락형성 |
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권병오1, 주혜영1, 김천수1, 전동석2, 김종인3, 김흥식4 |
1성균관대학교 의과대학 소아과학교실
2계명대학교 의과대학 임상병리학교실
3계명대학교 의과대학 산부인과학교실
4계명대학교 의과대학 소아과학교실 |
Correspondence:
Chun Soo Kim, Email: chunsoo7@unitel.co.kr
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| Abstract |
Purpose
: This study was undertaken to obtain basic data about the megakaryocyte colony formation of fetal liver cells by using immunocytochemical staining and ex vivo culture with growth factors.
Methods
: The mononuclear cells were isolated from fetal liver and bone marrow with idiopathic thrombocytopenic purpura(ITP) and pancytopenia. These mononuclear cells were cultured in MegaCultTM-C(Stem Cell Tech, Canada) media in the presence of growth factors and CFU-Megakaryocyte(CFU-Mk) colonies were counted on day 12. The expansion of CD34＋ and CD41＋ cell was analyzed by flow cytometry after 5 days incubation using flask culture.
Results
: The numbers of CFU-Mk colonies of mononuclear cells obtained from fetal liver in the 11th week gestational age were more than those in the 19th week specimens; growth factors could not enhance the colony expansion in all cases. Total numbers of CFU-Mk colony of fetal liver cells were higher than bone marrow from ITP or pancytopenia groups. The numbers of pure or large CFU-Mk colonies of fetal liver cells were also higher than bone marrow specimens. The rate of CD34＋ cell expression of fetal liver was increased after flask culture and the enhancement effect of epression was seen only in cases which added thrombopoietin. The rate of CD41＋ cell expression of fetal liver was increased after incubation, but the enhancement effect of growth factors was unclear.
Conclusion
: This study revealed good results about the megakaryocyte colony assay of fetal liver mononuclear cells using MegaCultTM-C media. This study suggests that the fetal liver could be a good source of megakaryocytic progenitor cells for clinical application in hematopoietic stem cell transplantation. |
| Key Words:
Hematopoietic stem cells, Fetal liver, CFU-Mk |
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