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Detection of Helicobacter pylori by Pre-embedding Immunoelectron Microscopy -Comparison with Immunoblotting Method

Journal of the Korean Pediatric Society 2002;45(7):862-874.
Published online July 15, 2002.
Detection of Helicobacter pylori by Pre-embedding Immunoelectron Microscopy -Comparison with Immunoblotting Method
Hae-Seoung Jung1, Eun-Jung Kim1, Eun-A Kim1, Ji-Hoe Park1, Jin-Su Jun1, Ji-Hyoun Seo1, Jae-Young Lim1, Myoung-Bum Choi1, Hyang-Ok Woo1, Hee-Shang Youn1, Gyung-Hyuck Ko2, Seung-Chul Baik3, Woo-Kon Lee3, Myung-Je Cho3, and Kwang-Ho Rhee3
1Departments of Pediatrics, Gyeongsang National University College of Medicine, Jinju, Korea
2Departments of Pathology, Gyeongsang National University College of Medicine, Jinju, Korea
3Departments of Microbiology, Gyeongsang National University College of Medicine, Jinju, Korea
Pre-embedding Immunoelectron Microscopy를 이용한 Helicobacter pylori의 발견- Immunoblotting법과 비교 -
정혜성1, 김은정1, 김은아1, 박지희1, 전진수1, 서지현1, 임재영1, 최명범1, 우향옥1, 윤희상1, 고경혁2, 백승철3, 이우곤3, 조명제3, 이광호3
1경상대학교 의과대학 소아과학교실
2경상대학교 의과대학 병리학교실
3경상대학교 의과대학 미생물학교실
Correspondence: 
Hee-Shang Youn, Email: hsyoun@nongae.gsnu.ac.kr
Abstract
Purpose
: We tried to evaluate whether the detection rate of Helicobacter pylori in gastric biopsy specimens could be improved by using pre-embedding immunoelectron microscopy.
Methods
: A total of 119 children who complained of upper gastrointestinal symptoms were endoscoped at the Gyeongsang National University Hospital from July, 1996 to July, 1999. Five biopsy specimens(three for urease test, one for hematoxylin-eosin(H & E) staining, and one for pre- embedding immunoelectron microscopy) were obtained from each antrum and body. Immunoblotting analysis were also performed.
Results
: Among the 119 patients, H. pylori were found in 116 patients(97.5%) by the immunoelectron microscopy. Among three patients who were found H. pylori negative in immunoelectron microscopy, two patients showed H. pylori in H & E stained slides and one patient was urease test positive(color change within six hours). Urease tests were positive in 107 patients(89.9 %). The positive rate of immunoblotting tests was 81.5%. However, only 13 patients(10.9%) showed H. pylori on the H & E stained antrum or body tissue.
Conclusion
: In this study, we found H. pylori histopathologically in most of the pediatric patients who complained of upper gastrointestinal symptoms. This study showed that pre-embedding immunoelectron microscopic examinations can be used as a gold standard in the diagnosis of childhood H. pylori infection. However, this method also has limited capacity to detect widely scattered H. pylori compared to the other histopathologic diagnostic methods.
Key Words: Helicobacter pylori, Pre-embedding immunoelectron microscopy, Diagnosis, Child


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