| The Morphologic Changes of Parvalbumin- Immunoreactive Interneurons of the Dentate Gyrus in Kainate-Treated Mouse
Hippocampal Slice Culture Epilepsy Model |
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Hee Sun Chung1, Mi-Young Shin1, Young-Hoon Kim1, In-Goo Lee1, Kyung-Tai Whang1, Myung-Suk Kim2 |
1Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea
2Department of Physiology, The Catholic University of Korea, Seoul, Korea |
| Kainic Acid로 처리한 해마박편배양 마우스 간질모델에서 치아이랑 Parvalbumin면역 반응성 사이신경세포의 형태학적 변화 |
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정희선1, 신미영1, 김영훈1, 이인구1, 황경태1, 김명석2 |
1가톨릭대학교 의과대학 소아과학교실
2가톨릭대학교 의과대학 생리학교실 |
Correspondence:
Young-Hoon Kim, Email: pedkyh@catholic.ac.kr
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| Abstract |
Purpose
: Loss of hippocampal interneurons in dentate gyrus has been reported in patients with severe temporal lobe epilepsy and in animals treated with kainic acid(KA). Interneurons contain Ca2＋- binding protein parvalbumin(PV). The effects of kainic acid on parvalbumin-immunoreactive (PV-IR) interneurons in dentate gyrus were investigated in organotypic hippocampal slice cultures.
Methods
: Cultured hippocampal slices from postnatal day nine C57/BL6 mice were exposed to 10 μM KA, and were observed at 0, 8, 24, 48, 72 hours after a one hour KA exposure. Neuronal injury was determined by morphologic changes of PV-IR interneuron in dentate gyrus.
Results
: Transient(1 hour) exposure of hippocampal explant cultures to KA produced marked varicosities in dendrites of PV-IR interneuron in dentate gyrus and the shaft of interbeaded dendrite is often much thinner than those in control. The presence of varicosities in dendrites was reversible with KA washout. The dendrites of KA treated explants were no longer beaded at 8, 24, 48 and 72 hours after KA exposure. The number of cells in PV-IR interneurons in dentate gyrus was decreased at 0, 8 hours after exposure. But there was no significant difference in 24, 48 and 72 hours recovery group compared with control group.
Conclusion
: The results suggested that loss of PV-IR interneurons in dentate gyrus is transient, and is not accompanied by PV-IR interneuronal cell death. |
| Key Words:
Kainic acid, Interneuron, Parvalbumin-immunoreactivity, Dentate gyrus |
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