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Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis

Journal of the Korean Pediatric Society 2003;46(3):271-276.
Published online March 15, 2003.
Plasma G-CSF and GM-CSF Concentrations and Expression of their Receptors on the Granulocyte in Children with Leukocytosis
Won Seok Choi, Kyung Hwan Ryu, You Jeong Kim, So Young Kim, Hyun Hee Kim, Wonbae Lee
Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea
백혈구 증가증 환아의 혈장내 G-CSF와 GM-CSF의 농도 및 과립구에서의 이들 수용체의 발현
최원석, 유경환, 김유정, 김소영, 김현희, 이원배
가톨릭대학교 의과대학 소아과학교실
Correspondence: 
Wonbae Lee, Email: lwb@hfh.cuk.ac.kr
Abstract
Purpose
: Granulocyte-colony stimulating factor(G-CSF) and granulocyte macrophage-colony stimulating factor(GM-CSF) are principal cytokines in granulopoiesis and their physiologic effects are mediated through binding to specific cell surface receptors. Although it is known that the level of serum G-CSF and GM-CSF, and presentation of the receptors are increased in infectious diseases, there have been no studies to find the correlation between the granulopoiesis and leukocytosis. This study was designed to measure G-CSF and GM-CSF in leukocytosis and in control and to demonstrate the possible pathogenesis of granulopoiesis in leukocytosis using quantitative analysis of G- CSF, GM-CSF and their CSFr.
Methods
: The plasma levels of G-CSF, GM-CSF of 13 children without leukocytosis and 14 children with leukocytosis were measured. Counts of cell surface G-CSFr and GM-CSFr were measured by combining anti G-CSFr and anti GM-CSFr monoclonal antibodies to their respective receptors by using quantitative flow cytometric assay.
Results
: There was no significant difference betweeen the plasma concentration of G-CSF and GM-CSF in acute leukocytosis and in the control group. However, levels of G-CSFr in acute leukocytosis decreased significantly compared to the control(P=0.012) and the levels of GM-CSFr in both groups revealed no significant difference.
Conclusion
: Increase in the number of leukocyte in leukocytosis was mediated by increasing the number of neutrophil, and increased plasma concentration of G-CSF may be the cause of neutrophilia. But GM-CSF did not have any influence on leukocytosis.
Key Words: Granulocyte-colony stimulating factor(G-CSF), Granulocyte macrophage-colony stimulating factor(GM-CSF), G-CSF receptor, GM-CSF receptor, Leukocytosis


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