| Differential Response of Surfactant Protein-A Genetic Variants to Dexamethasone Treatment |
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Eul Soon Kim1, In Kyu Lee1, Myung Ho Oh1, Chong Woo Bae2 |
1Department of Pediatrics, Soonchunhyang University Hospital, Chonan, Korea
2Department of Pediatrics, Kyunghee University Hospital, Seoul, Korea |
| 덱사메타손 처치에 따른 폐 표면 활성 단백질-A 유전자 변이의 반응의 차이점에 관한 연구 |
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김을순1, 이인규1, 오명호1, 배종우2 |
1순천향대학교 의과대학 소아과학교실
2경희대학교 의과대학 소아과학교실 |
Correspondence:
Myung Ho Oh, Email: omh@schch.co.kr
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| Abstract |
Purpose
: Surfactant protein A(SP-A) is involved in surfactant physiology and structure, and plays a major role in innate host defense and inflammatory processes in the lung. Steroid therapy is widely used for mothers who threaten to deliver prematurely and also used commonly in the management of preterm infants with chronic lung disease. Two SP-A genes(SP-A1, SP-A2) and several alleles have been characterized for each SP-A gene in human. Preliminary evidence indicates that differences may exist among alleles in response to Dexamethasone(Dexa) and that the SP-A 3'UTR plays a role in this process. We studied whether 3'UTR-mediated differences exist among the most frequently found SP-A alleles in response to Dexa.
Methods
: Constructs containing the 3'UTR from eight different SP-A alleles were made using luciferase as a the reporter gene. These constructs were driven by the SV40 promotor and were transfected along with a transfection control vector in H441 cells that express SP-A. The activity of the reporter gene in the presence or absence of Dexa(100 nM) treatment was measured. All the experiments for the eight SP-A alleles studied, were performed in triplicate and repeated five times. The results were normalized to the transfection control.
Results
: Expression of alleles of 6A3, 6A, 1A were significantly decreased in response to Dexa.
Conclusion
: Three UTR mediated differences exist among human SP-A variants both in the basal expression and in response to Dexa. These genotype-dependent differences may point to a need for a careful consideration of individual use of steroid treatment in the prematurely born infant. |
| Key Words:
Surfactant, Surfactant protein-A(SP-A), SP-A1 alleles, SP-A2 alleles, RDS, Steroid |
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