| Allele Distribution and Frequency of Human Surfactant
Protein-A2 in Korean Neonates |
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Nyeon Cheon Kim1, Hee Chul Yoon1, Jung Su Suk2, Jung Ho Ko2, Ook Joon Yoo2, In Kyu Lee1, Myung Ho Oh1, Chong Woo Bae3 |
1Department of Pediatrics, Soonchunhyang University Hospital, Chonan, Korea
2Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea
3Department of Pediatrics, Kyunghee University Hospital, Seoul, Korea |
| 한국 신생아의 폐 표면 활성제 단백-A2(Human SurfactantProtein-A2) 유전자 대립형질의 분포와 빈도 |
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김년천1, 윤희철1, 석정수2, 고정호2, 유욱준2, 이인규1, 오명호1, 배종우3 |
1순천향대학교 의과대학 소아과학교실
2한국과학기술원 생물과학과
3경희대학교 의과대학 소아과학교실 |
Correspondence:
Myung Ho Oh, Email: omh@schch.co.kr
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| Abstract |
Purpose
: We evaluated allele frequencies and distribution of surfactant protein A2(SP-A2) in Korean neonates in order to estimate the prevalence of RDS, to find out new SP-A alleles, and to establish new steroid therapy.
Methods
: Genomic DNA was extracted from 71 neonates and served as a template in PCR for genotype analysis. SP-A gene-specific amplications and gene-specific allele determinations were performed using PCR-cRFLP methods.
Results
: The distribution for the alleles of the SP-A2 gene in the study population was 1A, 1A0, 1A1, 1A2, 1A3, 1A5, 1A6, 1A7, 1A8, 1A9, 1A11, 1A12. The specific frequencies for the alleles of the SP- A2 gene in the study population were : 1A=11.3%, 1A0=38%, 1A1=12.7%, 1A2=9.2%, 1A5=15.5%, 1A7= 2.9%, 1A8=4.9%, 1A9=2.2%, others=3.3%.
Conclusion
: The frequency of 1A0 was higher than the other SP-A2 alleles in Korean neonates. This finding suggests that the prevalence of RDS in Korea may be low compared with other countries. However, this finding also suggests that Korean neonates have a high risk of infection. |
| Key Words:
Surfactant, SP-A2, SP-A2 alleles, RDS, Steroid |
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