| Bilirubin-Binding Capacity of Albumin in Korean Neonates. |
|
Jung Bae Lee1, Chul Lee1, Ran Namgung1, Dong Gwan Han1, Kyung Son Song2 |
1Department of Pediatrics, Yonsei University, College of Medicine, Seoul, Korea
2Department of Clinical Pathology, Yonsei University, College of Medicine, Seoul, Korea |
| 한국 신생아 혈청 알부민의 빌리루빈
결합능력에 관한 연구 |
|
이정배1, 이철1, 남궁란1, 한동관1, 송경순2 |
1연세대학교 의과대학 소아과학교실
2연세대학교 의과대학 임상병리학교실 |
|
Received: 6 March 1988 • Accepted: 19 May 1988 |
|
|
| Abstract |
|
The binding of bilirubin by albumin is thought to play an important role in the pathogenesis of
kemictems. Free bilirubin hypothesis has led to develop methods to evaluate the binding capacity and
affinity of bilirubin to albumin in the plasma. Horseradish peroxidase oxidation of free bilirubin can
estimate the level of free bilirubin, bilirubin binding capacity and bilirubin binding affinity.
Twenty four term infants and twenty adults were participated in this study. Serum free bilirubin
concentration was determined by automated peroxidase micromethod. Each serum was titrated for
the determination of bilirubin binding capacity and bilirubin binding affinity by serial additions of
small aliquots of bilirubin to the serum. Titration curves were plotted as free bilirubin versus total
bilirubin concentration. The titration data were plotted as Scatchard graphs to estimate the binding
capacity and binding affinity.
Free bilirubin concentration of neonatal serum were 3.76±1.20 n Mol/L.
Binding capacity of neonatal serum (25.55±3.95 mg%, or bilirubin: albumin molar ratios 0.87±0.15)
was significantly lower than that in adult serum (35.594-5.77 mg%; or blirubin: albumin molar ratios
1.05±0.14). Binding affinity of neonotal serum (8.81 ±2.65 X1O7/M) was significantly lower than that
in adult serum (17.83 ± 7.34 X107/M).
We concluded that the bilirubin binding ability of infant serum is less than that of adult serum.
Adult serum can bind bilirubin nearly twice as effectively as infant serum can. |
| Key Words:
Free Bilirubin, Bilirubin Binding Capacity, Bilirubin Binding Affinity |
|
PDF Links
Download Citation
