| Effects of 7-Nitroindazole on Brain Cell Membrane Function and Energy Metabolism during Transient Global Cerebral Hypoxia-Ischemia and Reoxygenation-Reperfusion in Newborn Piglets |
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Chang Won Choi, Jong Hee Hwang, Yun Sil Chang, Won Soon Park |
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Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea |
| 뇌의 저산소증-허혈과 재산소화-재관류가 유발된 신생자돈에서 7-Nitroindazole이 뇌세포막 기능과 에너지 대사에 미치는 영향에 대한 연구 |
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최창원, 황종희, 장윤실, 박원순 |
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성균관대학교 의과대학 삼성서울병원 소아과 |
Correspondence:
Won Soon Park, Email: wspark@smc.samsung.co.kr
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| Abstract |
Purpose
: Our study was undertaken to discover whether a selective neuronal nitric oxide synthase inhibitor, 7-nitroindazole, influences brain cell membrane function and energy metabolism during and after transient global hypoxia-ischemia(HI) in newborn piglets.
Methods
: Cerebral HI was induced by temporary complete occlusion of bilateral common carotid arteries and simultaneous breathing with 8% oxygen for 30 minutes, followed by release of carotid occlusion and normoxic ventilation for one hour(reoxygenation-reperfusion, RR). 7-Nitroindazole(50 mg/kg) or vehicle was administered intraperitoneally just before the induction of HI or RR. Brain cortex was harvested for the biochemical analysis at the end of HI or RR.
Results
: The level of conjugated dienes significantly increased and the activity of Na＋, K＋-ATPase significantly decreased during HI, and they did not recover during RR. The levels of ATP and phosphocreatine(PCr) significantly decreased during HI, and recovered during RR. 7-Nitroindazole did not influence significantly the level of conjugated dienes, the activity of Na＋, K＋-ATPase, and the levels of ATP and PCr during HI and RR.
Conclusion
: 7-nitroindazole did not demonstrate a neuroprotective effect in our piglet model of transient global cerebral HI and one hour of RR. |
| Key Words:
Hypoxia-ischemia, Brain, 7-Nitroindazole, Reperfusion injury, Metabolism, Animals, Newborn |
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