Korean Journal of Pediatrics 2004;47(2):204-209.
Published online February 15, 2004.
Effects of 7-Nitroindazole on Brain Cell Membrane Function and Energy Metabolism during Transient Global Cerebral Hypoxia-Ischemia and Reoxygenation-Reperfusion in Newborn Piglets
Chang Won Choi, Jong Hee Hwang, Yun Sil Chang, Won Soon Park
Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
뇌의 저산소증-허혈과 재산소화-재관류가 유발된 신생자돈에서 7-Nitroindazole이 뇌세포막 기능과 에너지 대사에 미치는 영향에 대한 연구
최창원, 황종희, 장윤실, 박원순
성균관대학교 의과대학 삼성서울병원 소아과
Won Soon Park, Email: wspark@smc.samsung.co.kr
: Our study was undertaken to discover whether a selective neuronal nitric oxide synthase inhibitor, 7-nitroindazole, influences brain cell membrane function and energy metabolism during and after transient global hypoxia-ischemia(HI) in newborn piglets.
: Cerebral HI was induced by temporary complete occlusion of bilateral common carotid arteries and simultaneous breathing with 8% oxygen for 30 minutes, followed by release of carotid occlusion and normoxic ventilation for one hour(reoxygenation-reperfusion, RR). 7-Nitroindazole(50 mg/kg) or vehicle was administered intraperitoneally just before the induction of HI or RR. Brain cortex was harvested for the biochemical analysis at the end of HI or RR.
: The level of conjugated dienes significantly increased and the activity of Na+, K+-ATPase significantly decreased during HI, and they did not recover during RR. The levels of ATP and phosphocreatine(PCr) significantly decreased during HI, and recovered during RR. 7-Nitroindazole did not influence significantly the level of conjugated dienes, the activity of Na+, K+-ATPase, and the levels of ATP and PCr during HI and RR.
: 7-nitroindazole did not demonstrate a neuroprotective effect in our piglet model of transient global cerebral HI and one hour of RR.
Key Words: Hypoxia-ischemia, Brain, 7-Nitroindazole, Reperfusion injury, Metabolism, Animals, Newborn

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