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The Effects of Intravenous Immunoglobulin(IVIG) and Methylprednisolone on the mRNAs Expressions of VEGF, VCAM-1 and IL-1β of Human Umbilical Vein Endothelial Cells(HUVEC) Stimulated by IL-1β

Korean Journal of Pediatrics 2004;47(12):1325-1333.
Published online December 15, 2004.
The Effects of Intravenous Immunoglobulin(IVIG) and Methylprednisolone on the mRNAs Expressions of VEGF, VCAM-1 and IL-1β of Human Umbilical Vein Endothelial Cells(HUVEC) Stimulated by IL-1β
Soh Yeon Kim, Sun Jeong Lim, Ji Whan Han, Kyung Yil Lee, Joon Sung Lee
Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea
정맥용 면역글로불린과 Methylprednisolone이 IL-1β로 자극된 인간제대정맥 내피세포에서 VEGF, VCAM-1,IL-1β mRNA의 발현에 미치는 영향
김소연, 임선정, 한지환, 이경일, 이준성
가톨릭대학교 의과대학 소아과학교실
Correspondence: 
Joon Sung Lee, Email: jslee@catholic.ac.kr
Abstract
Purpose
: Kawasaki disease(KD) manifests a systemic vasculitis of unknown etiology in young children. Vascular endothelial growth factor(VEGF), vascular cell adhesion molecule-1(VCAM-1) and interleukin-1 beta(IL-1β) may play important roles in the pathogenesis of KD. Intravenous immunoglobulin(IVIG) and methylprednisolone(MP) are therapeutically effective for KD, however, the precise mechanisms of the two drugs are still unknown. We investigated the therapeutic efficacy of IVIG and/or MP for KD in vitro.
Methods
: Human umbilical vein endothelial cells(HUVEC) obtained from umbilical cords of healthy newborns were cultured. After HUVEC were treated with IL-1β, the effect of IVIG and/or MP on the in vitro activation of HUVEC were assessed by cell proliferation and reverse transcription-polymerase chain reaction-detected expression of mRNA coding for VEGF, VCAM-1, and IL-1β.
Results
: IVIG and MP down-regulated the expression of VEGF mRNA induced by IL-1β(P<0.05, respectively) significantly. The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP compared to the IVIG or MP alone respectively(P<0.05). IVIG and MP down-regulated the expression of VCAM-1 mRNA induced by IL-1β(P<0.05, respectively). The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP(P<0.05). IVIG and MP down-regulated the expression of IL-1β mRNA induced by IL- 1β(P<0.001, P<0.05, respectively). The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP(P<0.001).
Conclusion
: These results suggested that IVIG and MP are therapeutically effective for KD in vitro as well as in vivo.
Key Words: Kawasaki disease, Human umbilical vein endothelial cells, Intravenous immunoglobulin, methylprednisolone, Vascular endothelial growth factor, Vascular cell adhesion molecule-1, Interleukin-1β


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