| Gene Expression of Metalloproteinases, Tissue Inhibitors of Metalloproteinases and Cytokines in Adriamycin-induced Cardiomyopathy |
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Young Mi Hong |
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Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea |
| 아드리아마이신으로 유도된 심근증에서 Metalloproteinase, Metalloproteinase 조직억제자, Cytokine 유전자 발현에 대한 연구 |
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홍영미 |
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이화대학교 의과대학 동대문병원 소아과 |
Correspondence:
Young Mi Hong, Email: hongym@chollian.net
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| Abstract |
ity has been reported to occur in both clinical and experimental forms of dilated cardiomyopathy. There was no report about MMP in adriamycin(ADR)-induced cardiomyopathy. The purpose of this study was to investigate gene expression of MMP and tissue inhibitor of metalloproteinases(TIMP) in ADR-induced cardiomyopathy and clarify the relationship between MMP and cytokines.
Methods
: Male Sprague-Dawley rats were divided into two groups. The first group was control. The second group was given intraperitoneal injections of ADR(5 mg/kg) twice a week over two weeks. Serum concentrations of MMP, TIMP, interleukin(IL)-6 and tumor necrosis factor(TNF)-α were measured. RNA extraction was performed from frozen rat hearts. Reverse transcription polymerase chain reaction(RT-PCR) was employed. cDNA Microarray analysis was performed by using a set of 5,184 sequence-verified rat cDNA clones.
Results
: Serum MMP and TIMP levels were not significantly different between the two groups. IL-6 was 36.8±2.8 pg/mL and TNF-α 2.2±2.7 pg/mL in the ADR group. They were significantly higher than in the control group. Serum MMP correlated significantly with TNF-α(r=0.41, P<0.05). There was no gene expression of MMP, IL-6 or TNF-α in the hearts of both groups. Gene expression of TIMP was significantly depressed in the hearts of the ADR group.
Conclusion
: These results suggested a potential role for TNF-α in the regulation of extracellular matrix remodeling in ADR induced cardiomyopathy. Rapid screening of multiple decreased gene expression by DNA chip may be a useful diagnostic test to detect early cardiac injury before developing ADR induced cardiomyopathy. |
| Key Words:
Gene, Metalloproteinases , Cytokines , Adriamycin |
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