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Ex vivo Expansion and Clonal Maintenance of CD34+ Selected Cells from Cord Blood and Peripheral Blood

Korean Journal of Pediatrics 2005;48(8):894-900.
Published online August 15, 2005.
Ex vivo Expansion and Clonal Maintenance of CD34+ Selected Cells from Cord Blood and Peripheral Blood
Soon Ki Kim1, Hye Yoon Ghil2, Sun U. Song2, Jong Weon Choi3, Sang Kyu Park4
1Department of Pediatrics , Inha University of Hospital, Inha Medical College, Incheon, Korea
2Clinical Research Center, , Inha University of Hospital, Inha Medical College, Incheon, Korea
3Department of Laboratory Medicine, Inha University of Hospital, Inha Medical College, Incheon, Korea
4Department of Pediatrics
제대혈 및 말포혈로부터 분리한 CD34 양성 세포의 체외 증폭 및 클론 유지
김순기1, 길혜윤2, 송순욱2, 최종원3, 박상규4
1인하대학교 의과대학 소아과학교실
2인하대학교병원 중앙연구소
3인하대학교병원 진단검사의학교실
4울산대학교 의과대학 소아과학교실
Correspondence: 
Sang Kyu Park, Email: sang@uuh.ulsan.kr
Abstract
Purpose
: Because of the unavailability of marrow transplantation, umbilical cord blood (CB) is increasingly being used. We evaluated the potential of ex vivo expansion and clonality in CD34+ cells separated from cord blood source and mobilized peripheral blood (PB) in a serum-free media.
Methods
: The CD34+ cells, selected from CB and mobilized PB, were expanded with hematopoietic growth factors. They were then cultured for burst-forming units of erythrocytes (BFU-E), colony- forming units of granulocytes and monocytes (CFU-GM) and colony-forming units of megakaryocytes (CFU-Mk) at culture days 0, day 4, day 7, and day 14 with various growth factors.
Results
: The CB-selected CD34+ cells showed significantly higher total cell expansion than those from the PB at day 7 (2 fold increase than PB). The CB-selected CD34+ cells produced more BFU-E colonies than did the PB on culture at days 7 and at day 14. Also, the CB-selected CD34+ cells produced more CFU-Mk colonies than did the PB on culture at day 4 and at day 7.
Conclusion
: The ex vivo expansion of the CB cells may be promising in producing total cellular expansion, CFU-Mk and BFU-E compared with PB for 7 to 14 days. The growth factors combination including megakaryocyte growth and development, flt3-ligand and interleukin-3 showed more expansion in the view of total cells and clonal maintenance compared with less combination.
Key Words: CD34+ cells , Colony-forming unit of megakaryocyte , Cord blood, Mobilized peripheral blood , Ex vivo expansion


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