| Effects of puromycin aminonucleoside on the cytoskeletal changes of
glomerular epithelial cells |
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Jun Ho Lee1, Tae Sun Ha2 |
1Department of Pediatrics, Pochon CHA University, College of Medicine
2Department of Pediatrics, Chungbuk National University, College of Medicine |
| Puromycin aminonucleoside의 사구체 상피세포에 대한 영향 |
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이준호1, 하태선2 |
1포천중문의대 분당차병원 소아청소년과
2충북대학교 의과대학 소아과학교실 |
Correspondence:
Tae Sun Ha, Email: tsha@chungbuk.ac.kr
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| Abstract |
of microvilli, but also separated the intercellular gaps and linear ZO-1. PAN induced oxidative stresses in time and dose dependent manners and increases of intercellular permeability in anti-oxidants inhibitable manners. High concentration of PAN induced not only actin polymerization and disorganization, but also the conglomerulation and internal dislocation of α-actinin protein. The intensities of fluorescences of ZO-1 protein were diminished and internalized by PAN in a dose-dependent manner, which were inhibited by anti anti-oxidants.
Conclusion
: PAN induced the changes of podocytes cytoskeleton and junctional barriers by way of increasing ROS in GEpC that resulted in increasing their permeability in a antioxidatn-inhibitable manner. Glomerular hyperpermeability induced by PAN mediateing through oxidative stresses is thought to take part in the mechanism of proteinuria in nephrotic syndrome. |
| Key Words:
Puromycin aminoglycoside, Nephrotic syndrome, Proteinuria, Oxidative stresses, Anti- oxidants |
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