| Polymorphisms of tumor necrosis factor-alpha promotor gene in Kawasaki disease and relation to the risk of coronary artery lesion |
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Se-hwa Kim, Jang-won Yun, Young-hyuk Lee, Eun-jung Cheon |
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Department of Pediatrics, College of Medicine, Konyang University, Daejeon, Korea |
| 가와사키병에서의 TNF-alpha 유전자의 다형성 및 관상동맥 합병증과의 연관성 |
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김세화, 윤장원, 이영혁, 천은정 |
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건양대학교 의과대학 소아과학교실 |
Correspondence:
Eun-jung Cheon, Email: koojook@medimail.co.kr
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| Abstract |
Purpose
: The purpose of this study was to investigate the polymorphisms of the TNF-alpha promotor gene, its susceptibility to Kawasaki disease (KD) and to assess whether the TNF-alpha promotor gene polymorphism was related the risk of coronary artery lesions (CALs).
Methods
: From January 2003 to January 2007, 51 children (30 boys and 21 girls) with KD and 48 children forming an age-matched control group were studied. DNA from the peripheral blood of all the children was sampled, and the DNA polymorphisms of the 5' flanking regions of the TNF-alpha promoter gene at position -308 [guanine (G) to adenine (A)] were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Then, the relationship between KD and the TNF-alpha promotor gene polymorphisms was evaluated.
Results
: The A allele frequency of the -308 site of the TNF-alpha promotor gene was 17.6% (9/51) for children with KD and 6.8% (3/48) for the control group children, but this result was not statistically significant. Twenty-four patients experienced CALs within 60 days after the onset of symptoms. KD children with TNF-alpha -308 A allele had lower frequencies of CALs (12.5% versus 22.2%, P>0.05).
Conclusion
: The DNA polymorphism of the -308 site TNF-alpha gene was not associated with susceptibility to KD and a risk of CALs. Multicenter, large-scale randomized controlled trials are needed for further study. |
| Key Words:
Mucocutaneous lymph node syndrome, Coronary arteries, TNF-alpha polymorphism |
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