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Nitroblue Tetrazolium Reduction by Pseudoeosinophils from Rabbits Treated with Bacterial or Viral Vaccine.

Journal of the Korean Pediatric Society 1979;22(8):671-680.
Published online August 15, 1979.
Nitroblue Tetrazolium Reduction by Pseudoeosinophils from Rabbits Treated with Bacterial or Viral Vaccine.
Sang Ghern Chung, Jong Wo Shin
Department of Pediatrics, College of Medicine Busan National University, Korea.
세균성 또는 바이러스성 백신으로 처리한 토끼의 위호산구에 의한 Nitroblue Tetrazolium 의 환원
鄭相建, 申鍾雨
부산대학교 의과대학 소아과학교실
Abstract
This study was undertaken to acquire some information concerning the mechanism for reduction of nitroblue tetrazolium (NBT) dye by neutrophils. Male rabbits weighing more than 2 kilograms were used in this study. The vaccines. The vaccines utilized were bacterial and viral ones such as typhoid, cholera, measles, and mumps vaccines. The histochemical NBT test was carried out using the method by Park et al. With some modification. Vaccines were given the rabbits, and changes were observed in the percentage and number of pseudoeosinophils and NBT-positive pseudoeosils in the peripheral blood. The data obtained thus were discussed and summarized as follow:1. The percentage of the NBT-positive pseudoeosinophils increased in the rabbits to which the bacterial vaccines were given. 2. The percentage of NBT-positive pseudoeosinophils decreased in the rabbits to whick viral vaccines were given. 3. No association was found between the percentage of the NBT-positive pseudoeosinophils and the number of pseudodosinophils following the administeration.


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