Breastfeeding and vitamin D

Ju Sun Heo; Young Min Ahn; Ai-Rhan Ellen Kim; Son Moon Shin

doi:10.3345/cep.2021.00444

Clin Exp Pediatr > Volume 65(9); 2022

Heo, Ahn, Kim, Shin, and for the Korean Society of Breastfeeding Medicine: Breastfeeding and vitamin D

Review Article

Neonatology (Perinatology)

Breastfeeding and vitamin D

Ju Sun Heo, MD, PhD¹

, Young Min Ahn, MD, PhD²

, Ai-Rhan Ellen Kim, MD, PhD³

, Son Moon Shin, MD, PhD⁴

;for the Korean Society of Breastfeeding Medicine

¹Department of Pediatrics, Anam Hospital, Korea University College of Medicine, Seoul, Korea

²Department of Pediatrics, Jang’s Hospital, Seoul, Korea

³Department of Pediatrics, Ulsan University College of Medicine, Seoul, Korea

⁴Department of Pediatrics, Inje University Busan Paik Hospital, Inje University College of Medicine, Busan, Korea

Corresponding author: Young Min Ahn, MD, PhD. Department of Pediatrics, Jang’s Hospital, 411, Mangu-ro, Jungnang-gu, Seoul 02073, Korea Email: ymahn964@naver.com

Received April 12, 2021 Revised October 9, 2021 Accepted November 15, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Clinical and Experimental Pediatrics 2022;65(9):418-429.

Published online: December 14, 2021

DOI: https://doi.org/10.3345/cep.2021.00444

Abstract

The recent re-emergence of vitamin D deficiency (VDD) and rickets among breastfed infants without adequate sunlight exposure and vitamin D supplementation has been reported worldwide. Breastfed infants are particularly vulnerable to VDD because of the low vitamin D content of breast milk, restricted sunlight exposure, increased pollution, and limited natural dietary sources of vitamin D. The prevalence of VDD in breastfed infants differs vastly between studies and nations at 0.6%–91.1%. The recommended intake of vitamin D for lactating mothers to optimize their overall vitamin D status and, consequently, of their breast milk is 200–2,000 IU/day, indicating a lack of consensus. Some studies have suggested that maternal high-dose vitamin D supplementation (up to 6,400 IU/day) can be used as an alternate strategy to direct infant supplementation. However, concern persists about the safety of maternal high-dose vitamin D supplementation. Direct infant supplementation is the currently available option to support vitamin D status in breastfed infants. The recommended dose for vitamin D supplementation in breastfed infants according to various societies and organizations worldwide is 200–1,200 IU/day. Most international guidelines recommend that exclusively or partially breastfed infants be supplemented with 400 IU/day of vitamin D during their first year of life. However, domestic studies on the status and guidelines for vitamin D in breastfed infants are insufficient. This review summarizes the prevalence of VDD in breastfed infants, vitamin D content of breast milk, and current guidelines for vitamin D supplementation of lactating mothers and infants to prevent VDD in breastfed infants.

Key words: Vitamin D, Breastfeeding, Infant

Key message

∙ Exclusively breastfed infants are at risk of developing vitamin D deficiency associated with hypocalcemia, rickets, and various health outcomes.

∙ The prevalence of vitamin D deficiency in breastfed infants differs vastly between studies and nations at 0.6%–91.1%.

∙ The vitamin D content of breast milk does not meet the requirements of exclusively breastfed infants.

∙ Most international guidelines recommend that breastfed infants be supplemented with 400 IU/day of vitamin D during the first year of life.

∙ Vitamin D intake (milk+supplements) of 800 IU/day can be considered in preterm infants along with biochemical monitoring.

Graphical abstract

Introduction

Vitamin D (calciferol), a fat-soluble steroid hormone, is important for regulating calcium homeostasis and bone health [1]. Moreover, the importance of vitamin D is emphasized as evidence supports an association between vitamin D and other health outcomes, such as immune function, respiratory diseases, allergy, olfactory function, cardiovascular diseases, obesity, insulin resistance, and cancer in pediatric and adolescent populations [2-24].

Vitamin D exists in 2 forms: vitamin D₃ (cholecalciferol), the mammalian form; and vitamin D₂ (ergocalciferol), the fungal form [25]. In vivo, vitamin D₃ can be synthesized in the epidermis through photochemical transformation of 7-dihydrocholesterol after exposure to ultraviolet radiation B (wavelength 280–320 nm) energy [26-29]. Vitamin D₂ and D₃ can also be obtained through dietary intake; both follow the same metabolic pathways, and their actions are qualitatively similar [30]. Vitamin D is converted by 25-hydroxylase to 25-hydroxyvitamin D (25(OH)D) in the liver and further metabolized to the biologically active form 1,25-dihydroxyvitamin D through 1α-hydroxylation action in the kidney. Sunlight exposure often has a major influence on vitamin D status, accounting for over 90% of its level [31]. However, skin synthesis of vitamin D is negatively influenced by a dark skin color, living in northern latitudes during winter, use of sunscreen with a high Sun Protection Factor, body covering, air pollution, and staying indoors for much of the day [32,33]. In comparison, the dietary intake of vitamin D, in addition to fortified foods, has little impact on an individual’s overall vitamin D status [33].

Breast milk is an important source of energy and nutrients for children until 2 years of age. To ensure optimal infant growth, development, and health, the World Health Organization (WHO) recommends exclusive breastfeeding for the first 6 months of life and the introduction of nutritionally adequate and safe complementary foods at 6 months together with continued breastfeeding up to 2 years of age or beyond [34]. However, human milk typically contains a small amount of vitamin D (approximately ≤25–50 IU/L) [1,25,35], and the concentration is affected by the lactating mother’s vitamin D status and season [36,37]. It is well known that a smaller amount of vitamin D is supplied to infants by lactating mothers with vitamin D deficiency (VDD). Therefore, exclusively breastfed infants are more likely to develop VDD, resulting in hypocalcemia and rickets if sunlight exposure is limited [30,38]. Vitamin D plays an important role in bone mineralization as well as the regulation of skeletal muscle function and the immune, nervous, and cardiovascular systems [39-42]. Therefore, it is very important to maintain an appropriate vitamin D level while continuing to breastfeed.

This review aims to examine the relationship between breast feeding and vitamin D and review guidelines for vitamin D supplementation in lactating mothers and infants to prevent VDD in breastfed infants.

Definition of deficiency, insufficiency, and sufficiency of vitamin D

In the blood, 25(OH)D has a long half-life of 2–3 weeks and does not need to be strictly controlled to maintain homeostasis. Therefore, it is a good indicator of the level of vitamin D intake (cutaneous synthesis and dietary intake) [36,43]. On the other hand, 1,25-dihydroxyvitamin D has a short half-life of 5–8 hours and is always maintained at a constant blood level in healthy people [44]. The use of serum levels of 1,25-dihydroxyvitamin D is limited to monitoring certain conditions, such as acquired and inherited disorders of vitamin D and phosphate metabolism [45]. Guidelines for VDD/insufficiency/sufficiency defined by serum 25(OH)D concentrations have been published by many societies in many countries [25,30,32,33,45-51]. These guidelines are summarized in Table 1. According to published guidelines, a 25(OH)D level of ≤12–20 ng/mL is deficient, while a level of ≥20–30 ng/mL is sufficient.

In South Korea, there are no consensus guidelines regarding the definition of VDD/insufficiency or sufficiency based on 25(OH)D levels. In the 2020 Dietary Reference Intakes (DRIs) for Koreans, the optimal 25(OH)D level for bone health was determined to fall within the range that minimizes parathyroid hormone secretion and maximizes calcium absorption [36]. Based on previous studies, a 25(OH)D level of 20–30 ng/mL is considered the cut-off level that satisfies these conditions [52,53]. The clinical guidelines of the Korean Society of Pediatric Endocrinology do not recommend additional vitamin D supplementation when the 25(OH)D level is greater than 20 ng/mL [54]. However, there are insufficient domestic data to support this suggestion.

Vitamin D status of breastfed infants

There are increasing reports of VDD among breastfed infants who do not receive vitamin D supplementation [55]. The vitamin D statuses of exclusively or nearly exclusively breastfed infants and their mothers are shown in Table 2. The prevalence of VDD differs greatly between studies and nations at 0.6%–91.1% [56-66]. Among these studies, 7 (63.6%) reported a prevalence of VDD of 50% or higher in breastfed infants. A prevalence as high as 83% was reported among 1-month-old infants in Qatar despite the region having abundant sunshine [61]. One study in India reported a prevalence of VDD of 91.1% among 6-month-old infants who were not on vitamin D supplementation [58]. VDD in infants exclusively breastfed without vitamin D supplementation and sufficient sun exposure is a major cause of nutritional rickets, with a high prevalence in North America, South America, Europe, and parts of the Middle East [67].

In South Korea, a few studies have reported on the vitamin D status of breastfed infants (Table 2) [68-71]. The prevalence of VDD varied among the studies according to definitions of VDD. In studies conducted before 2010, VDD was defined as a 25(OH)D level of <11 ng/mL. Park et al. [71] found that the prevalence of VDD was 44% among breastfed infants, higher than the 6% among formula-fed infants. Kim et al. [70] reported that concentrations of vitamin D were measured at 0, 6, and 12 months. Among breastfed infants without supplementation, the prevalence of VDD gradually decreased from birth (26.9%) to 12 months of age (0%). In studies published after 2013, however, the prevalence of VDD increased up to 81%–90.4% [68,69]. This increase may have been caused by changes in the definition of VDD as 25(OH)D level <20 ng/mL, although the possibility of an increase in actual VDD infants cannot be ruled out.

Based on previous studies, the prevalence of VDD is high in exclusively breastfed infants, even in South Korea. Further large-scale domestic studies are necessary to evaluate the exact vitamin D status of breastfed infants.

Vitamin D content of breast milk

According to studies conducted in the 1980s, breast milk contains both vitamin D and 25(OH)D [72,73]. Vitamin D and 25(OH)D, known for their antirachitic activity (ARA; expressed in IU/L), are responsible for ≥90% of the total vitamin D activity in breast milk [73]. The dihydroxylated metabolites did not contribute significantly to the total vitamin D activity. ARA is calculated as 1 IU/L=25 pg/mL vitamin D=5 pg/mL vitamin 25(OH)D [74]. A very small amount (approximately 1%) of the 25(OH)D circulating in the maternal blood crosses into the breast milk [75]; in contrast, 20%–30% of the circulating vitamin D is expressed in the breast milk [76]. However, vitamin D is rapidly converted to 25(OH)D; therefore, the concentration of vitamin D circulating in the maternal blood decreases rapidly unless recent sunlight exposure and adequate vitamin D supplementation are provided.

Previous studies reported that the ARA levels in the breast milk of Western mothers were 14–170 IU/L (Table 3) [72-74,77-79]. However, there is a dearth of studies on vitamin D content of the breast milk of Asian mothers. A recent study compared vitamin concentrations of the breast milk of women from South Korea, China, Pakistan, and Vietnam [80]. However, the concentrations of vitamin D₂ and D₃ in the breast milk samples from all 4 countries were lower than the limit of detection of D₂=0.5 ng/mL and D₃=1 ng/mL. Three studies in Japan evaluated vitamin D concentrations of breast milk. One study compared the vitamin D concentrations of breast milk between 1989 and 2016–2017 [37]. The mean total ARA of breast milk from 1989 to 2016–2017 decreased from 31.3 IU/L to 25.5 IU/L, respectively. In the other 2 studies from Japan, the mean ARA of vitamin D₃ and 25(OH)D₃ were 3.2–3.5 IU/L and 16.2 IU/L, respectively [81,82]. The study conducted in Malaysia reported higher 25(OH)D levels (80.8–100.8 IU/L) than those reported in Japanese studies [83].

On combining studies from different countries, the levels of vitamin D of breast milk did not meet the vitamin D requirements of exclusively breastfed infants despite the mothers being exposed to abundant lifetime sunlight [74].

Effects of vitamin D supplementation of lactating mothers on vitamin D status and bone health of breastfed infants

The vitamin D content of breast milk is related to the vitamin D status of lactating mothers [74,84-87]. Lactating mothers with VDD would have a reduced amount of vitamin D in their breast milk, resulting in VDD in breastfed infants [88]. Therefore, there have been some efforts to supply adequate vitamin D to the nursing infants through maternal vitamin D supplementation.

The dietary recommendations of vitamin D for lactating mothers differ among advisory bodies, ranging from 200 to 2,000 IU/day [25,33,45,46,51,89,90]. The Institute of Medicine suggested 600 IU/day for lactating mothers [25], whereas the Endocrine Society suggested 1,500–2,000 IU/day [45]. Consensus is lacking on the recommended intake of vitamin D for lactating mothers, and the supplemental vitamin D dose that optimizes the vitamin D status of mothers and breast milk is unclear. In South Korea, the 2020 DRIs for Koreans recommended vitamin D supplementation of 400 IU/day for lactating mothers [36]. In previous studies, however, the breast milk of a mother receiving a vitamin D dose of 400 IU/day contains very low vitamin D concentrations of approximately 25–80 IU/L, which is far below the recommended daily vitamin D intake for infants [84,87,91,92]. The WHO panel acknowledged that supplementation during lactation to improve the vitamin D content of breast milk may require a maternal intake much higher than 600 IU/day [91,93-95].

A recent meta-analysis evaluated the effects of maternal vitamin D supplementation on the vitamin D status and bone health of term breastfed infants [96]. It included 5 randomized controlled trials (RCTs) comparing supplementation with placebo or no treatment [58,62,97-99]. These studies reported a mean vitamin D level of 266 for vitamin D-supplemented mothers and 246 for controls, and the converted daily doses of vitamin D were 1,785–4,000 IU/day, higher than the current recommended dose. The results showed that high-dose vitamin D supplementation of lactating mothers may increase the 25(OH) D level (mean difference, 24.62 nmol/L; 95% confidence interval [CI], 21.59–27) [60]. in their children and reduce the incidence of vitamin D insufficiency (risk ratio [RR], 0.47; 95% CI, 0.39–0.57), VDD (RR, 0.15; 95% CI, 0.09–0.24), and biochemical rickets (RR, 0.06; 95% CI, 0.01–0.44) in breastfed infants compared with placebo or no treatment [96]. However, there was insufficient evidence of the effects on bone mineral content and radiological rickets.

Studies have focused on methods of maternal supplementation to achieve vitamin D–replete milk to avoid direct infant supplementation. Wagner et al. [87] found that the supplementation of lactating women with 6,400 IU/day of vitamin D for 6 months significantly improved maternal vitamin D status and increased the ARA of the breast milk from 82 IU/L to 873 IU/L. The mean plasma 25(OH)D level in exclusively breastfed infants was 46 ng/mL, similar to that of the unsupplemented mothers’ infants receiving 300 IU/day of vitamin D. Maternal and infant urinary calcium-to-creatinine ratios remained within the reference range for both groups. This was confirmed by a large RCT in which maternal intake of 6,400 IU/day resulted in a supply of breast milk with adequate vitamin D to satisfy their nursing infants’ requirements [100]. Maternal high-dose supplementation (6,400 IU/day) resulted in similar 25(OH)D levels in infants supplemented with 400 IU/day. Another RCT recently evaluated the effects of intermittent mega-dose of vitamin D on lactating mothers in India [58]. In this study, mothers received either vitamin D₃ 60,000 IU at 24–48 hours postpartum and at 6, 10, and 14 weeks amounting to 240,000 IU of vitamin D₃ or placebo. They reported that the intervention groups had higher serum 25(OH)D concentrations in exclusively breastfed infants and a 94.6% and 48.1% reduction in the risk of VDD and vitamin D insufficiency, respectively, at 6 months of age. Neither the mother nor the infant experienced adverse effects.

However, concern persists about maternal high-dose vitamin D supplementation despite the lack of adverse effects. These high vitamin D doses are above the recommended upper limit of 4,000 IU/day for lactating mothers [25,101]. The global consensus recommendations highlight that lactating women should not consume high amounts of vitamin D to supplement their infants [33]. The clinical protocol of the Academy of Breastfeeding Medicine recommends maternal high-dose supplementation only if direct infant supplementation is contraindicated [102].

Thus far, the final consensus is that supplementing both mothers and infants with vitamin D, rather than supplementing mothers alone with high-dose vitamin D, ensures a healthy vitamin D status in their infants.

Guidelines for vitamin D supplementation in breastfed infants

Direct infant supplementation is the currently available option to improve vitamin D levels. This supplement should be vitamin D₃ because of its superior absorption compared to vitamin D₂ [102]. The recommended dose for vitamin D supplementation in breastfed infants is available from various societies and organizations worldwide and differs among them (Table 4). The WHO provides general recommendations for specific vulnerable groups. The WHO recommends 200 IU/day of vitamin D for infants aged <1 year [89].

In 2003, the American Academy of Pediatrics (AAP) recommended that all infants, including those who are exclusively breastfed, should have a minimum intake of 200 IU/day of vitamin D beginning in the first 2 months of life [35]. In 2008, the AAP, with the primary intention of preventing rickets considering new evidence from clinical trials, increased the recommended intake of vitamin D for infants aged <1 year from 200 IU/day to 400 IU/day beginning soon after birth [103]. The guidelines stated that exclusively or partially breastfed infants should be supplemented with 400 IU/day of vitamin D. For nonbreastfed infants ingesting <1,000 mL/day of vitamin D-fortified formula or milk, the AAP recommended vitamin D supplementation of 400 IU/day as well [103]. In 2011, the Institute of Medicine (now known as the National Academy of Medicine) agreed with this recommendation [25], and the AAP, through an endorsement of the Institute of Medicine report in 2012 [104] and a clinical report in 2014 [48], re-emphasized its original 2008 recommendation.

Most European countries as well as the Unites States recommend vitamin D supplementation with at least 400 IU/day for infants during the first year of life [46]. The European Society for Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) also stated that all infants should receive oral supplementation of 400 IU/day of vitamin D. However, the Committee on Nutrition of the French Society of Pediatrics recommended higher doses of vitamin D [105]. The recommendations were as follows: 1,000–1,200 IU/day for breastfed infants, 600–800 IU/day for children ≤18 months of age receiving milk supplemented with vitamin D; and 1,000–1,200 IU for children less than 18 months of age receiving milk not supplemented with vitamin D. Closely followed supplementation of breastfed infants with 1,000 IU/day of vitamin D has helped prevent the resurgence of rickets in France [105].

In Japan, the DRIs were revised in 2020 [106]. The Japanese DRIs are influenced by tentative dietary goals to prevent lifestyle-related diseases. Adequate intake (AI) in infants was defined as the vitamin D concentration in the breast milk multiplied by the typical intake of 0.78 L/day. However, considering the evidence of the re-emergence of VDD rickets and low content of vitamin D of breast milk [107], the AI was set at 200 IU/day for both male and female infants aged <1 year [106].

In South Korea, there are no national guidelines regarding vitamin D intake in breastfed infants. The 2020 DRIs for Koreans recommend a dose of 200 IU/day of vitamin D as the AI for infants, similar to the 2015 DRIs for Koreans [36]. The Korean Society of Pediatric Endocrinology suggested 400–600 IU/day of vitamin D supplementation for infants, children, and adolescents [54] based on the guidelines of the Institute of Medicine and the AAP [25,48]. It is necessary to develop Korean guidelines for vitamin D supplementation based on evidence from domestic studies on vitamin D levels in the blood considering sunlight exposure and food intake.

Most international guidelines recommend 400 IU/day of vitamin D for infants aged <1 year after birth. A recent meta-analysis showed that vitamin D supplementation of 400 IU/day may increase the 25(OH)D level (mean difference, 22.63 nmol/L; 95% CI, 17.05–28.21) and may reduce the incidence of vitamin D insufficiency [25(OH)D] (RR, 0.57; 95% CI, 0.41–0.80) compared with placebo or no treatment among term breastfed infants [96]. However, there is insufficient evidence of the effect of supplementation on VDD and bone health.

Vitamin D supplementation for preterm infants

Preterm infants are at a high risk of VDD for several reasons: (1) decreased transplacental transfer, (2) minimal vitamin D formation mediated by ultraviolet B due to prolonged hospitalization, (3) minimal fat mass in which vitamin D and its metabolites are stored, and (4) a relatively high vitamin D requirement [108-110]. A lack of vitamin D supplementation in the early postnatal period is a risk factor for metabolic bone disease in preterm infants [111,112]. Therefore, supplementation with an optimal dose of vitamin D is very important for preterm infants.

The AAP recommends vitamin D intake of 200–400 IU/day for very low birth weight infants (birth weight <1,500 g) (Table 5) [108]. For preterm infants with a birth weight ≥1,500 g, the recommended intake of vitamin D was 400 IU/day with a maximum upper level of 1,000 IU/day, similar to the recommended intake for full-term infants. Meanwhile, the ESPGHAN guidelines generally suggest a daily vitamin D intake of 800–1,000 IU/day for preterm infants up to a weight of approximately 1,800 g and at least 400 IU/day for late and moderately preterm infants [110,113].

Several RCTs published after 2011 compared the efficacy of low-dose (200–400 IU/day) and high-dose (800–1,000 IU/day) supplementation of vitamin D for preterm infants (Table 5). In all studies, serum 25(OH)D levels after supplementation were significantly higher in the high-dose versus low-dose group [114-119]. The prevalence of VDD was also significantly lower in the high-dose group [114,116,118,120]. Two studies showed that the high-dose group experienced bone density benefits [117,120]. One study reported that the high-dose group had better growth results [120], consistent with the results of a recent meta-analysis [121]. Despite the benefits of high-dose supplementation, concern persists about the adverse effects of excess vitamin D intake, such as hypercalciuria or nephrocalcinosis [114,116,122]. The risk of vitamin D overdose could be increased upon achieving a total dose of 1,000 IU/day, combining supplements and diets, especially in neonates with a birth weight <1,000 g [123].

Based on the results of the trials mentioned above, a vitamin D intake of 800 IU/day can be considered in preterm infants along with biochemical monitoring. The total vitamin D intake of 800 IU/day should include the vitamin D content of human milk fortifier or formula as well as vitamin D supplementation. The vitamin D contents of human milk fortifiers and formulas commonly fed in Korea are summarized in Table 6.

Three sources of vitamin D

1. Sun exposure

Ultraviolet radiation B from sun exposure is a major source of vitamin D. Several studies reported a positive correlation between sun exposure and serum vitamin D levels in breastfed infants [124,125]. Meena et al. [125] suggested a minimum 30-minute weekly afternoon sunlight exposure between 10:00 AM and 3:00 PM over 40% of the body area (infant clothed in a diaper in the prone position) for at least 16 weeks to achieve sufficient vitamin D levels by 6 months of age [125]. However, despite intense sun exposure, 90% of infants continued to have vitamin D insufficiency and required supplementation [126].

The harmful effects of ultraviolet rays must also be considered despite their beneficial effects associated with vitamin D synthesis. It is known to increase the risk of photodamage and melanoma or non-melanoma skin cancer, especially in the Caucasian population [127]. The AAP recommends that infants younger than 6 months be kept out of direct sunlight [128,129]. Outdoor activities should be planned to minimize peak-intensity midday sun exposure (10:00 AM to 4:00 PM) [128]. Current guidelines recommend that vitamin D requirements be met through supplementation rather than deliberate sun exposure in infants [127,128].

2. Food sources of vitamin D

Vitamin D is present in a preactive form in several foods. Foods rich in vitamin D are summarized in Table 6. Vitamin D₃ is found in foods of animal origin, such as blue fatty fish, egg yolk, liver, and milk. Vitamin D₂ is found in small amounts in food of vegetable origin and wild mushrooms [130].

3. Vitamin D supplementation

As mentioned earlier, 400 IU/day of vitamin D supplementation for breastfed infants is recommended by most international guidelines. This supplement should be cholecalciferol, vitamin D₃, due to its superior absorption [102]. However, there were major concerns about parental compliance [131], pediatrician practices [63], and incorrect dosing risk [132,133]. In 2020, a systematic review reported on infant intermittent vitamin D supplementation as an alternative to daily supplementation [134]. The doses varied from a single bolus of 30,000–600,000 IU, an intermittent bolus of 50,000 IU×3 to 600,000 IU×2, and 1,400 IU/wk from postnatal 7 days to 6 months. Overall, bolus dosing (>50,000 IU) achieves higher 25(OH)D repletion rates earlier than daily dosing, but it is likely to have similar efficacy at preventing VDD in later infancy. Considering the findings of trials to date, the evidence remains too weak to recommend intermittent vitamin D supplementation as an alternative to daily supplementation.

Conclusion

Breastfed infants are vulnerable to VDD because of the low vitamin D content of breast milk, restricted sunlight exposure, and limited access to natural dietary sources of vitamin D. These infants are at major risk for VDD rickets, which appears to have re-emerged on a global scale. Most international guidelines recommend that exclusively or partially breastfed infants be supplemented with 400 IU/day of vitamin D during their first year of life. However, domestic studies of the status and guidelines for vitamin D in breastfed infants are insufficient. Therefore, further research with a larger number of subjects is needed to clarify the status of VDD in Korean infants and guidelines to ensure adequate vitamin D status.

Footnotes

Conflicts of interest

No potential conflict of interest relevant to this article was reported.

Funding

This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Table 1.

Definitions of deficiency, insufficiency, and sufficiency of vitamin D according to clinical guidelines

Society/organization	Year	25-hydroxyviamin D (ng/mL^a))
Society/organization	Year	Deficiency	Insufficiency	Sufficiency
Institute of Medicine [25]	2011	<12	12-19	≥20
The Endocrine Society [45]	2011	<20	21–29	≥30
European Society for Pediatric Gastroenterology Hepatology and Nutrition [32]	2013	<20	-	≥20
Central Europe [46]	2013	<20	20–29	≥30
Society for Adolescent Health and Medicine [47]	2013	<20	20–29	≥30
American Academy of Pediatrics [48]	2014	<20	-	≥20
European Food Safety Authority [49]	2016	-	-	≥20
Global Consensus for Rickets [33]	2016	<12	12–19	≥20
Japanese Society for Bone and Mineral Research, Japan Endocrine Society [30]	2017	<20	20–29	≥30
United Arab Emirates [50]	2018	<20	20–29	≥30
Korean Society of Pediatric Endocrinology [54]	2019	-	-	≥20
Australasia Paediatric Endocrine Group Bone and Mineral Working Group [51]	2020	<12	12–19	≥20

^a) 1 ng/mL=2.5 nmol/L.

Table 2.

Vitamin D statuses of exclusively breastfed infants and their mothers

First author, year	Country	No. of participants	Age of infants	25(OH)D (ng/mL) of mothers^a)	25(OH)D (ng/mL) of infants^a)	Prevalence of VDD in infants	Remark
Parian-de los Angeles et al., 2021 [56]	Philippines	131 Infants	<6 Mo	-	-	VDD (<15 ng/mL): 77%
Parian-de los Angeles et al., 2021 [56]	Philippines	131 Infants	<6 Mo	-	-	VDI (15-20 ng/mL): 10%
Said et al., 2020 [57]	Kenya	98 Infants	-	-	-	VDD (<12 ng/mL): 11.2%
Said et al., 2020 [57]	Kenya	98 Infants	-	-	-	VDI (12–20 ng/mL): 12.2%
Trivedi et al., 2020 [58]	India	56 M-I pairs	6 Mo	6.29±4.11	6.43±3.76	VDD (<11 ng/mL): 91.1%	Control group
Trivedi et al., 2020 [58]	India	56 M-I pairs	6 Mo	6.29±4.11	6.43±3.76	VDI (11–19 ng/mL): 8.9%	Control group
Terashita et al., 2017 [59]	Japan	9 Infants	5 Mo	-	16.0±6.5	VDD (<20 ng/mL): 67%	Vitamin D supplementation (-)
Haugen et al., 2016 [60]	Nepal	500 M-I pairs	1–12 Mo	21.2±8.1	34.8±7.6	VDD (<12 ng/mL): 0.6%	BM (full or partly)
Haugen et al., 2016 [60]	Nepal	500 M-I pairs	1–12 Mo	21.2±8.1	34.8±7.6	VDI (12–19 ng/mL): 3.0%	BM (full or partly)
Salameh et al., 2016 [61]	Arab in Doha, Qatar	60 M-I pairs	1 Mo	13 (10–18)	8 (5–13.5)	VDD (<20 ng/mL): 83%
Wheeler et al., 2016 [62]	New Zealand	30 M-I pairs	5 Mo	-	-	VDD (<20 ng/mL): 27%	71% exc BM at 5 mo, control group
Dawodu et al., 2014 [63]	USA	120 M-I pairs	4 Wk	28.1±9.3	16.4±6.5	VDD (<20 ng/mL): 77%	87.5% exc BM at 4 weeks
Wall et al., 2013 [64]	New Zealand	94 Infants	2–3 Mo	-	21.2 (5.6–40)	VDD (<11 ng/mL): 24%
Jain et al., 2011 [65]	India	98 M-I pairs	2.5–3.5 Mo	9.8 (5.0–13.8)	10.1 (2.5–17.1)	VDD (<15 ng/mL): 66.7%	70.6% exc BM
Jain et al., 2011 [65]	India	98 M-I pairs	2.5–3.5 Mo	9.8 (5.0–13.8)	10.1 (2.5–17.1)	VDI (15–20 ng/mL): 19.8%	70.6% exc BM
Agarwal et al., 2010 [66]	India	97 M-I pairs	10 Wk	9.34±6.18	11.55±7.27	VDD (<11 ng/mL): 55.67%
Kang et al., 2015 [68]	South Korea	70 M-I pairs	4–24 Mo	12.4±4.4	11.4±8.6	VDD (≤20 ng/mL): 81%
Choi et al., 2013 [69]	South Korea	52 Infants	1–6 Mo	-	9.35	VDD (<20 ng/mL): 90.4%	Feeding BM>80% of total feeding volume/day
Kim et al., 2010 [70]	South Korea	28 M-I pairs	0–12 Mo	24.5±12.5	0 → 6 → 12 mo: 18.0±10.3 → 22.5±9.24 → 33.2±7.89	0 → 6 → 12 mo: VDD (<11 ng/mL)	Exc BM without supplementation
						26.9% → 10.7% → 0%
						VDI (11–30 ng/mL)
						61.5% → 67.9% → 50.0%
Park et al., 1998 [71]	South Korea	18 Infants	2–5 Mo	-	16.0±11.3	VDD (<11 ng/mL) 44%

25(OH)D, 25-hydroxyvitamin D; VDD, vitamin D deficiency; VDI, vitamin D insufficiency; BM, breast milk; exc BM, exclusive breast milk; M-I, mother-infant.

^a) Values are presented as mean±standard deviations or median (interquartile range).

Table 3.

Antirachitic activity of breast milk in examined studies

Study	Year	Country	ARA of vitamin D and 25(OH)D in breast milk^*
Hollis et al., [72]	1981	USA	Vitamin D: 1.56±0.36 IU/L
Hollis et al., [72]	1981	USA	25(OH)D: 62.2±6.2 IU/L
Reeve et al., [73]	1982	USA	Vitamin D2 and D3: 14–16 IU/L
Reeve et al., [73]	1982	USA	25(OH)D: 33 IU/L
Ala-Houhala et al., [77]	1988	Finland	124 (19–332) IU/L in summer, 14 (8–30) IU/L in winter
Sakurai et al., [81]	2005	Japan	Vitamin D3: 3.2±4.3 IU/L
Kamao et al., [82]	2007	Japan	Vitamin D3: 3.5 IU/L
Kamao et al., [82]	2007	Japan	25(OH)D3: 16.2 IU/L
Jan Mohamed et al., [83]	2014	Malaysia	25(OH)D: 80.8–100.8 IU/L
við Streym et al., [78]	2016	Denmark	Vitamin D
			Foremilk in winter: 1.6 (1.6–6.4) IU/L
			Foremilk in summer: 6.4 (1.6–20.8) IU/L
			Hindmilk in winter: 4.8 (1.6–14.4) IU/L
			Hindmilk in summer: 14.4 (4.8–48) IU/L
			25(OH)D
			Foremilk in winter: 64 (40–88) IU/L
			Foremilk in summer: 72 (56–120) IU/L
			Hindmilk in winter: 96 (64–128) IU/L
			Hindmilk in summer: 128 (88–176) IU/L
Stoutjesdijk et al., [74]	2017	Netherland	46 (3–51) IU/L
		Curaçao	31 (5–113) IU/L
		Vietnam	Halong Bay 58 (23–110) IU/L
			Phu Tho 28 (1–62) IU/L
			Tien Giang 63 (26–247) IU/L
			Ho–Chi–Minh–City 49 (24–116) IU/L
			Hanoi 37 (11–118) IU/L
		Malaysia	Kuala Lumpur 14 (1–46) IU/L
		Tanzania	Ukerewe 77 (12–232) IU/L
		Tanzania	Maasai 88 (43–189) IU/L
Oberson et al., [79]	2020	USA	33.4–172.3 IU/L
Tsugawa et al., [37]	2021	Japan	31.3±30.3 IU/L in 1989, 25.5±12.0 IU/L in 2016–2017

Values are presented as mean±standard deviations or median (range).

25(OH)D, 25-hydroxyvitamin D.

^* ARA (calculated antirachitic activity)=biological activity of 25(OH)D+biological activity of vitamin D. Biological activity of vitamin D, 1 IU/L=25 pg/mL; biological activity of 25(OH)D, 1 IU/L=5 pg/mL.

Table 4.

Dietary recommended intakes (IU) of vitamin D for infants and children

Society/organization	Age 0–1 yr	Age 1–3 yr	Age 4–10 yr
Worldwide (WHO/FAO, 2014) [135]	200	200	200
Global consensus, 2016 [33]	400	600	600
USA (Endocrine Society, 2011) [45]	400–1,000	600–1,000	600–1,000
USA (IOM, 2011) [25]	400	600	600
USA (AAP, 2014) [48]	400	600	600
France (French Society of Pediatrics, 2012) [105]	1,000–1,200	<18 mo: fortified milk 600–800, not fortified milk 1,000–1,200	<5 yr: 2 × 80,000–100,000 (1 Nov and 1 Feb)
France (French Society of Pediatrics, 2012) [105]	1,000–1,200	≥18 mo: 2 × 80,000–100,000 (1 Nov and 1 Feb)	≥5 yr: (-)
Central Europe (Polish Scientific Committee on Vitamin D, 2013) [46]	400-600	600–1,000	600–1000
Europe (ESPGHAN, 2013) [32]	400	-	-
Japan (DRIs, 2020) [106]	200	Male: 120–140	Male: 140–200
Japan (DRIs, 2020) [106]	200	Female: 140–160	Female: 160–240
Korea (KSPE, 2019) [54]	400	600	600
Korea (DRIs by MOHW and KNS, 2020) [36]	200	200	200

WHO/FAO, World Health Organization/Food and Agriculture Organization; IOM, Institute of Medicine; AAP, American Academy of Pediatrics; ESPGHAN, European Society for Pediatric Gastroenterology Hepatology and Nutrition; KSPE, Korean Society of Pediatric Endocrinology; DRIs, dietary reference intakes; MOHW, Ministry of Health and Welfare; KNS, Korean Nutrition Society.

Table 5.

Summary of recommendations and randomized controlled trials of different doses of vitamin D for preterm infants

Study	Population	Recommendations
AAP, 2013 [108]	<1,500 g	200–400 IU/day
AAP, 2013 [108]	≥1,500 g	400–1,000 IU/day
ESPGHAN, 2010 [110]	<1,800 g	800–1,000 IU/day
ESPGHAN, 2019 [113]	32–36 weeks	At least 400 IU/day
Study	Population	No. of participants according to the groups	Findings
Natarajan et al., 2014 [114]	28–34 Weeks	800 IU (n=48) vs. 400 IU (n=48)	800 IU (vs. 400 IU)
			- VDD at 40 weeks’ PMA↓ (38.1% vs. 66.7%)
			- VDD at 3 month’s CA ↓ (12.5% vs. 35%)
			- Bone mineral content, bone mineral density →
			- Vitamin D excess (100–150 ng/mL): 1 (2.4%) in the 800 IU group
Fort et al., 2016 [115]	23–27 Weeks	800 IU (n=30) vs. 200 IU (n=34) vs. placebo (n=36)	800 IU (vs. 200 IU vs. placebo)
			- 25(OH)D at day 28 ↑ (84.5 ng/mL vs. 39 ng/mL vs. 22 ng/mL)
			- VDD (0% vs. 16% vs. 41%, P=0.2)
			- days alive, respiratory outcomes →
Mathur et al., 2016 [120]	<1,500 g	1,000 IU (n=25) vs. 400 IU (n=25)	1,000 IU (vs. 400 IU)
			- Serum 25(OH)D after 6 weeks of supplementation ↑ (50.9 ng/mL vs. 29.7 ng/mL)
			- VDD ↓ (0% vs. 20%)
			- Calcium ↑, ALP ↓, parathyroid hormone ↓
			- Skeletal hypomineralization ↓, growth ↑
Tergestina et al., 2016 [116]	27–34 Weeks	1,000 IU (n=60) vs. 400 IU (n=60)	1,000 IU (vs 400 IU)
			- VDD at 40 weeks’ PMA ↓ (2% vs 64.6%)
			- 25(OH)D at 40 weeks’ PMA ↑ (47.47 ng/mL vs. 17.48 ng/mL)
			- Parathyroid hormone ↓, calcium →, phosphorus →, ALP →
			- Vitamin D excess (>70 ng/mL): 9.8% of 1,000 IU group, but not associated with clinical or biochemical evidence of toxicity
			- Hypercalcemia →, elevated urine calcium/creatinine →
Anderson-Berry et al., 2017 [117]	24–32 Weeks	800 IU (n=16) vs. 400 IU (n=16)	800 IU (vs. 400 IU)
			- 25(OH)D at 4 weeks ↑ (42.12 ng/mL vs. 33.84 ng/mL)
			- DEXA bone density measurements <10p ↓ (16% vs 56%)
Bozkurt et al., 2017 [118]	24–32 Weeks	1,000 IU (n=40) vs. 800 IU (n=41) vs. 400 IU (n=40)	1,000 IU (vs. 800 IU vs. 400 IU)
			- 25(OH)D at 36 weeks’ PMA ↑ (43 ng/mL vs. 40 ng/mL vs. 29.4 ng/mL)
			- VDD at 36 weeks’ PMA compared to 400 IU ↓ (2.5% vs. 22.5%)
			- Vitamin D insufficiency at 36 weeks’ PMA compared to 400 IU ↓ (12% vs. 23%)
			- Calcium, phosphorus, ALP, parathyroid hormone →
			- Urine calcium/creatinine →
Abdel-Hady et al., 2019 [119]	28–36 Weeks with LOS	800 IU (n=25) vs. 400 IU (n=25)	800 IU (vs 400 IU)
			- VDD at 40 weeks’ PMA →
			- 25(OH)D at 40 weeks’ PMA ↑ (67.4 ng/mL vs. 54.8 ng/mL)
			- IL-6, TNF-α, growth, duration of oxygen and respiratory support, duration of antimicrobial use, length of hospital stay, mortality →
			- Vitamin D toxicity (-)

AAP, American Academy of Pediatrics; ESPGHAN, European Society for Pediatric Gastroenterology Hepatology and Nutrition; VDD, vitamin D deficiency; PMA, postmenstrual age; CA, corrected age; 25(OH)D, 25-hydroxyvitamin D; ALP, alkaline phosphatase; DEXA, dual-energy x-ray absorptiometry; IL, interleukin; LOS, late-onset sepsis; TNF, tumor necrosis factor.

↑, significantly higher in the high-dose group; ↓, significantly lower in the high-dose group; →, no significant differences between groups.

Table 6.

Vitamin D contents of formulas and foods

Formula and foods	IU per serving
Human milk fortifier, 1 pack	30–37.5
Preterm formula, 100 mL	56–128
Term formula, 100 mL	33.2–52
Cod liver oil, 1 table spoon (1 table spoon=15 mL)	1,360
Salmon, cooked, 100 g	360
Mackerel, cooked 100 g	345
Sardine, oiled and canned, 100 g	270
Eel, cooked, 100 g	200
Milk, vitamin D fortified, 200 mL	100
Egg, 1 large (vitamin D is found in yolk)	25
Mushroom, 100 g	20

Source of vitamin D contents of foods: National Health Information Portal, Korea Disease Control and Prevention Agency. http://health.kdca.go.kr/healthinfo.

References

1. Thorne-Lyman A, Fawzi WW. Vitamin D during pregnancy and maternal, neonatal and infant health outcomes: a systematic review and meta-analysis. Paediatr Perinat Epidemiol 2012;26:75–90.

2. Xiao J, He W. The immunomodulatory effects of vitamin D drops in children with recurrent respiratory tract infections. Am J Transl Res 2021;13:1750–6.

3. Panfili FM, Roversi M, D’Argenio P, Rossi P, Cappa M, Fintini D. Possible role of vitamin D in COVID-19 infection in pediatric population. J Endocrinol Invest 2021;44:27–35.

4. Knihtilä HM, Stubbs BJ, Carey VJ, Laranjo N, Chu SH, Kelly RS, et al. Low gestational vitamin D level and childhood asthma are related to impaired lung function in high-risk children. J Allergy Clin Immunol 2021;148:110. –9. e9.

5. Sharif A, Haddad Kashani H, Sharif MR. Association of 25-hydroxy vitamin D with asthma and its severity in children: a case-control study. Clin Mol Allergy 2020;18:7.

6. Singh N, Kamble D, Mahantshetti NS. Effect of vitamin D supplementation in the prevention of recurrent pneumonia in under-five children. Indian J Pediatr 2019;86:1105–11.

7. Shin YH, Ha EK, Kim JH, Yon DK, Lee SW, Sim HJ, et al. Serum vitamin D level is associated with smell dysfunction independently of aeroallergen sensitization, nasal obstruction, and the presence of allergic rhinitis in children. Pediatr Allergy Immunol 2021;32:116–23.

8. Chen YS, Mirzakhani H, Lu M, Zeiger RS, O'Connor GT, Sandel MT, et al. The association of prenatal vitamin D sufficiency with aeroallergen sensitization and allergic rhinitis in early childhood. J Allergy Clin Immunol Pract 2021;9:3788–96.e3.

9. Briceno Noriega D, Savelkoul HFJ. Vitamin D and allergy susceptibility during gestation and early life. Nutrients 2021;13:1015.

10. Tiazhka OV, Selska ZV. Application of vitamin D in different dosage to treat children with allergic diseases. Wiad Lek 2020;73:1377–83.

11. Cristi F, Perez-Mateluna G, Vera-Kellet C, Silva-Valenzuela S, Iturriaga C, Hoyos-Bachiloglu R, et al. Vitamin D modulates the allergic phenotype of dendritic cells in children with atopic dermatitis. Exp Dermatol 2019;28:308–11.

12. Cai B, Luo X, Zhang P, Luan Y, Cai X, He X. Effect of vitamin D supplementation on markers of cardiometabolic risk in children and adolescents: a meta-analysis of randomized clinical trials. Nutr Metab Cardiovasc Dis 2021;31:2800–14.

13. Xie H, Min M, Guo S, Xian Y, Yang F, Wang X, et al. Impact of vitamin D and vitamin D receptor on risk of cardiovascular diseases in children and adolescents with obesity in Sichuan, China: a cross-sectional study. Ann Nutr Metab 2020;76:396–404.

14. Milagres LC, Filgueiras MS, Rocha NP, Suhett LG, de Albuquerque FM, Juvanhol LL, et al. Cutoff point estimation for serum vitamin D concentrations to predict cardiometabolic risk in Brazilian children. Eur J Clin Nutr 2020;74:1698–706.

15. Lopez-Mayorga A, Hauger H, Petersen RA, Vogel U, Damsgaard CT, Lauritzen L. Vitamin D-related genes and cardiometabolic markers in healthy children: a Mendelian randomisation study. Br J Nutr 2020;123:1138–47.

16. Hauger H, Laursen RP, Ritz C, Mølgaard C, Lind MV, Damsgaard CT. Effects of vitamin D supplementation on cardiometabolic outcomes in children and adolescents: a systematic review and meta-analysis of randomized controlled trials. Eur J Nutr 2020;59:873–84.

17. Garfein J, Flannagan KS, Gahagan S, Burrows R, Lozoff B, Villamor E. Vitamin D status in infancy and cardiometabolic health in adolescence. Am J Clin Nutr 2020;113:104–12.

18. Colak R, Anil M, Yasar F, Rahmi Bakiler A, Pirgon O, Helvaci M, et al. Metabolic disturbances and cardiovascular risk factors in obese children with vitamin D deficiency. Arch Pediatr 2020;27:140–5.

19. Censani M, Hammad HT, Christos PJ, Schumaker T. Vitamin D deficiency associated with markers of cardiovascular disease in children with obesity. Glob Pediatr Health 2018;5:2333794. x17751773.

20. McClorry S, Slupsky CM, Lind T, Karlsland Åkeson P, Hernell O, Öhlund I. Effectiveness of vitamin D supplementation in Swedish children may be negatively impacted by BMI and serum fructose. J Nutr Biochem 2020;75:108251.

21. Flores Ruelas Y, Del Toro Equihua M, Alejandra Jiménez Solís N, Baltazar Rodríguez LM, Delgado Enciso I, Sánchez Ramírez CA. Vitamin D status and its relation to insulin resistance in a Mexican pediatric population. J Pediatr Endocrinol Metab 2020;33:481–6.

22. Corica D, Zusi C, Olivieri F, Marigliano M, Piona C, Fornari E, et al. Vitamin D affects insulin sensitivity and β-cell function in obese nondiabetic youths. Eur J Endocrinol 2019;181:439–50.

23. Jackmann N, Mäkitie O, Harila-Saari A, Gustafsson J, Nezirevic Dernroth D, Frisk P. Vitamin D status in children with leukemia, its predictors, and association with outcome. Pediatr Blood Cancer 2020;67:e28163.

24. Revuelta Iniesta R, Rush R, Paciarotti I, Rhatigan EB, Brougham FHM, McKenzie JM, et al. Systematic review and meta-analysis: prevalence and possible causes of vitamin D deficiency and insufficiency in pediatric cancer patients. Clin Nutr 2016;35:95–108.

25. Institute of Medicine (US) Committee to Review Dietary Reference Intakes for Vitamin D and Calcium. Dietary reference intakes for calcium and vitamin D. In: Ross AC, Taylor CL, Yaktine AL, Del Valle HB, editors. Dietary reference intakes for calcium and vitamin D. Washington, DC: National Academies Press (US), 2011.

26. Holick MF, Clark MB. The photobiogenesis and metabolism of vitamin D. Fed Proc 1978;37:2567–74.

27. Bikle DD, Nemanic MK, Gee E, Elias P. 1,25-Dihydroxyvitamin D₃ production by human keratinocytes. Kinetics and regulation. J Clin Invest 1986;78:557–66.

28. Bär M, Domaschke D, Meye A, Lehmann B, Meurer M. Wavelength-dependent induction of CYP24A1-mRNA after UVB-triggered calcitriol synthesis in cultured human keratinocytes. J Invest Dermatol 2007;127:206–13.

29. DeLuca HF. Overview of general physiologic features and functions of vitamin D. Am J Clin Nutr 2004;80:1689S–1696S.

30. Okazaki R, Ozono K, Fukumoto S, Inoue D, Yamauchi M, Minagawa M, et al. Assessment criteria for vitamin D deficiency/insufficiency in Japan - proposal by an expert panel supported by Research Program of Intractable Diseases, Ministry of Health, Labour and Welfare, Japan, The Japanese Society for Bone and Mineral Research and The Japan Endocrine Society [Opinion]. Endocr J 2017;64:1–6.

31. Paxton GA, Teale GR, Nowson CA, Mason RS, McGrath JJ, Thompson MJ, et al. Vitamin D and health in pregnancy, infants, children and adolescents in Australia and New Zealand: a position statement. Med J Aust 2013;198:142–3.

32. Braegger C, Campoy C, Colomb V, Decsi T, Domellof M, Fewtrell M, et al. Vitamin D in the healthy European paediatric population. J Pediatr Gastroenterol Nutr 2013;56:692–701.

33. Munns CF, Shaw N, Kiely M, Specker BL, Thacher TD, Ozono K, et al. Global consensus recommendations on prevention and management of nutritional rickets. J Clin Endocrinol Metab 2016;101:394–415.

34. World Health Organization. Infant and young child feeding. Geneva (Switzerland): WHO; 2020 [cited 2021 Mar 8]. Available from: https://www.who.int/en/news-room/fact-sheets/detail/infant-and-young-childfeeding.

35. Gartner LM, Greer FR. Prevention of rickets and vitamin D deficiency: new guidelines for vitamin D intake. Pediatrics 2003;111:908–10.

36. The Korean Nutrition Society. 2020 Dietary Reference Intakes for Korean [Internet]. Seoul (Korea): The Korean Nutrition Society; 2020 [cited 2021 Mar 10]. Available from: http://www.kns.or.kr/FileRoom/FileRoom_view.asp?idx=108&BoardID=Kdr.

37. Tsugawa N, Nishino M, Kuwabara A, Ogasawara H, Kamao M, Kobayashi S, et al. Comparison of Vitamin D and 25-hydroxyvitamin D concentrations in human breast milk between 1989 and 2016-2017. Nutrients 2021;13:573.

38. Wagner CL, Hollis BW. Early-Life Effects of Vitamin D: a focus on pregnancy and lactation. Ann Nutr Metab 2020;76 Suppl 2:16–28.

39. Halfon M, Phan O, Teta D. Vitamin D: a review on its effects on muscle strength, the risk of fall, and frailty. Biomed Res Int 2015;2015:953241.

40. Mailhot G, White JH. Vitamin D and immunity in infants and children. Nutrients 2020;12:1233.

41. Wrzosek M, Łukaszkiewicz J, Wrzosek M, Jakubczyk A, Matsumoto H, Piątkiewicz P, et al. Vitamin D and the central nervous system. Pharmacol Rep 2013;65:271–8.

42. Michos ED, Cainzos-Achirica M, Heravi AS, Appel LJ. Vitamin D, calcium supplements, and implications for cardiovascular health: JACC focus seminar. J Am Coll Cardiol 2021;77:437–49.

43. Saggese G, Vierucci F, Boot AM, Czech-Kowalska J, Weber G, Camargo CA Jr, et al. Vitamin D in childhood and adolescence: an expert position statement. Eur J Pediatr 2015;174:565–76.

44. Bae YJ, Kratzsch J. Vitamin D and calcium in the human breast milk. Best Pract Res Clin Endocrinol Metab 2018;32:39–45.

45. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, et al. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J Clin Endocrinol Metab 2011;96:1911–30.

46. Płudowski P, Karczmarewicz E, Bayer M, Carter G, Chlebna-Sokół D, Czech-Kowalska J, et al. Practical guidelines for the supplementation of vitamin D and the treatment of deficits in Central Europe - recommended vitamin D intakes in the general population and groups at risk of vitamin D deficiency. Endokrynol Pol 2013;64:319–27.

47. Society for Adolescent Health and Medicine. Recommended vitamin D intake and management of low vitamin D status in adolescents: a position statement of the Society for Adolescent Health and Medicine. J Adolesc Health 2013;52:801–3.

48. Golden NH, Abrams SA. Optimizing bone health in children and adolescents. Pediatrics 2014;134:e1229. –43.

49. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA). Dietary reference values for vitamin D. EFSA J 2016;14:4547.

50. Haq A, Wimalawansa SJ, Pludowski P, Anouti FA. Clinical practice guidelines for vitamin D in the United Arab Emirates. J Steroid Biochem Mol Biol 2018;175:4–11.

51. Siafarikas A, Simm P, Zacharin M, Jefferies C, Lafferty AR, Wheeler BJ, et al. Global consensus on nutritional rickets: implications for Australia. J Paediatr Child Health 2020;56:841–6.

52. Rosen CJ. Clinical practice. Vitamin D insufficiency. N Engl J Med 2011;364:248–54.

53. Mithal A, Wahl DA, Bonjour JP, Burckhardt P, Dawson-Hughes B, Eisman JA, et al. Global vitamin D status and determinants of hypovitaminosis D. Osteoporos Int 2009;20:1807–20.

54. The Korean Society of Pediatric Endocrinology Clinical Guidelines. Clinical practice guidelines for bone health in children and adolescents 2019. Seoul (Korea): Korean Society of Pediatric Endocrinology, 2019.

55. Dawodu A, Tsang RC. Maternal vitamin D status: effect on milk vitamin D content and vitamin D status of breastfeeding infants. Adv Nutr 2012;3:353–61.

56. Parian-de los Angeles E, Retoriano K, Arnaldo H, Ronquillo-Nolasco ME, Urtula R. Vitamin D status of breastfed Filipino infants aged less than 6 months in an urban community. Pediatr Gastroenterol Hepatol Nutr 2021;24:403–12.

57. Said NA, Kamenwa RW, Limbe MS, Okumu MO, Macharia WM. Prevalence of vitamin D deficiency in exclusively breastfed infants at a tertiary healthcare facility in Nairobi, Kenya. Arch Endocrinol Metab 2021;64:726–34.

58. Trivedi M, Faridi MMA, Aggarwal A, Madhu SV, Malhotra RK. Oral vitamin D supplementation to mothers during lactation-effect of 25(OH) D concentration on exclusively breastfed infants at 6 months of age: a randomized double-blind placebo-controlled trial. Breastfeed Med 2020;15:237–45.

59. Terashita S, Nakamura T, Igarashi N. Longitudinal study on the effectiveness of vitamin D supplements in exclusively breast-fed infants. Clin Pediatr Endocrinol 2017;26:215–22.

60. Haugen J, Ulak M, Chandyo RK, Henjum S, Thorne-Lyman AL, Ueland PM, et al. Low prevalence of vitamin D insufficiency among Nepalese infants despite high prevalence of vitamin D insufficiency among their mothers. Nutrients 2016;8:825.

61. Salameh K, Al-Janahi NS, Reedy AM, Dawodu A. Prevalence and risk factors for low vitamin D status among breastfeeding mother-infant dyads in an environment with abundant sunshine. Int J Womens Health 2016;8:529–35.

62. Wheeler BJ, Taylor BJ, Herbison P, Haszard JJ, Mikhail A, Jones S, et al. High-dose monthly maternal cholecalciferol supplementation during breastfeeding affects maternal and infant vitamin D status at 5 months postpartum: a randomized controlled trial. J Nutr 2016;146:1999–2006.

63. Dawodu A, Zalla L, Woo JG, Herbers PM, Davidson BS, Heubi JE, et al. Heightened attention to supplementation is needed to improve the vitamin D status of breastfeeding mothers and infants when sunshine exposure is restricted. Matern Child Nutr 2014;10:383–97.

64. Wall CR, Grant CC, Jones I. Vitamin D status of exclusively breastfed infants aged 2-3 months. Arch Dis Child 2013;98:176–9.

65. Jain V, Gupta N, Kalaivani M, Jain A, Sinha A, Agarwal R. Vitamin D deficiency in healthy breastfed term infants at 3 months & their mothers in India: seasonal variation & determinants. Indian J Med Res 2011;133:267–73.

66. Agarwal N, Faridi MM, Aggarwal A, Singh O. Vitamin D status of term exclusively breastfed infants and their mothers from India. Acta Paediatr 2010;99:1671–4.

67. Creo AL, Thacher TD, Pettifor JM, Strand MA, Fischer PR. Nutritional rickets around the world: an update. Paediatr Int Child Health 2017;37:84–98.

68. Kang YS, Kim JH, Ahn EH, Yoo EG, Kim MK. Iron and vitamin D status in breastfed infants and their mothers. Korean J Pediatr 2015;58:283–7.

69. Choi YJ, Kim MK, Jeong SJ. Vitamin D deficiency in infants aged 1 to 6 months. Korean J Pediatr 2013;56:205–10.

70. Kim MJ, Na B, No SJ, Han HS, Jeong EH, Lee W, et al. Nutritional status of vitamin D and the effect of vitamin D supplementation in Korean breast-fed infants. J Korean Med Sci 2010;25:83–9.

71. Park MJ, Namgung R, Kim DH, Tsang RC. Bone mineral content is not reduced despite low vitamin D status in breast milk-fed infants versus cow's milk based formula-fed infants. J Pediatr 1998;132:641–5.

72. Hollis BW, Roos BA, Draper HH, Lambert PW. Vitamin D and its metabolites in human and bovine milk. J Nutr 1981;111:1240–8.

73. Reeve LE, Chesney RW, DeLuca HF. Vitamin D of human milk: identification of biologically active forms. Am J Clin Nutr 1982;36:122–6.

74. Stoutjesdijk E, Schaafsma A, Nhien NV, Khor GL, Kema IP, Hollis BW, et al. Milk vitamin D in relation to the 'adequate intake' for 0-6-month-old infants: a study in lactating women with different cultural backgrounds, living at different latitudes. Br J Nutr 2017;118:804–12.

75. Thandrayen K, Pettifor JM. Maternal vitamin D status: implications for the development of infantile nutritional rickets. Rheum Dis Clin North Am 2012;38:61–79.

76. Taylor SN, Wagner CL, Hollis BW. Vitamin D supplementation during lactation to support infant and mother. J Am Coll Nutr 2008;27:690–701.

77. Ala-Houhala M, Koskinen T, Parviainen MT, Visakorpi JK. 25-Hydroxyvitamin D and vitamin D in human milk: effects of supplementation and season. Am J Clin Nutr 1988;48:1057–60.

78. við Streym S, Højskov CS, Møller UK, Heickendorff L, Vestergaard P, Mosekilde L, et al. Vitamin D content in human breast milk: a 9-mo follow-up study. Am J Clin Nutr 2016;103:107–14.

79. Oberson JM, Bénet S, Redeuil K, Campos-Giménez E. Quantitative analysis of vitamin D and its main metabolites in human milk by supercritical fluid chromatography coupled to tandem mass spectrometry. Anal Bioanal Chem 2020;412:365–75.

80. Nguyen MTT, Kim J, Lee H, Won S, Kim Y, Jung JA, et al. A comparison of vitamin and lutein concentrations in breast milk from four Asian countries. Nutrients 2020;12:1794.

81. Sakurai T, Furukawa M, Asoh M, Kanno T, Kojima T, Yonekubo A. Fatsoluble and water-soluble vitamin contents of breast milk from Japanese women. J Nutr Sci Vitaminol (Tokyo 2005;51:239–47.

82. Kamao M, Tsugawa N, Suhara Y, Wada A, Mori T, Murata K, et al. Quantification of fat-soluble vitamins in human breast milk by liquid chromatography–tandem mass spectrometry. J Chromatog B 2007;859:192–200.

83. Jan Mohamed HJ, Rowan A, Fong B, Loy SL. Maternal serum and breast milk vitamin D levels: findings from the Universiti Sains Malaysia Pregnancy Cohort Study. PLoS One 2014;9:e100705.

84. Cancela L, Le Boulch N, Miravet L. Relationship between the vitamin D content of maternal milk and the vitamin D status of nursing women and breast-fed infants. J Endocrinol 1986;110:43–50.

85. Basile LA, Taylor SN, Wagner CL, Horst RL, Hollis BW. The effect of high-dose vitamin D supplementation on serum vitamin D levels and milk calcium concentration in lactating women and their infants. Breastfeed Med 2006;1:27–35.

86. Hollis BW, Wagner CL. Assessment of dietary vitamin D requirements during pregnancy and lactation. Am J Clin Nutr 2004;79:717–26.

87. Wagner CL, Hulsey TC, Fanning D, Ebeling M, Hollis BW. High-dose vitamin D₃ supplementation in a cohort of breastfeeding mothers and their infants: a 6-month follow-up pilot study. Breastfeed Med 2006;1:59–70.

88. Wagner C. Current concepts in vitamin D requirements for mother and her breastfeeding infant. Breastfeed Med 2012;7:561–2.

89. WHO (World Health Organization). Vitamin and mineral requirements in human nutrition, 2nd ed. Geneva (Switzerland): WHO, 2004.

90. Nordic Council of Ministers, Nordic Council of Ministers Secretariat. Nordic Nutrition Recommendations 2012: Integrating nutrition and physical activity. 5th ed. Copenhagen (Denmark): Nordisk Ministerråd, 2014;:627.

91. Hollis BW, Wagner CL. Vitamin D requirements during lactation: highdose maternal supplementation as therapy to prevent hypovitaminosis D for both the mother and the nursing infant. Am J Clin Nutr 2004;80:1752S–8S.

92. Specker BL, Tsang RC, Hollis BW. Effect of race and diet on human-milk vitamin D and 25-hydroxyvitamin D. Am J Dis Child 1985;139:1134–7.

93. Schoenmakers I, Pettifor JM, Peña-Rosas JP, Lamberg-Allardt C, Shaw N, Jones KS, et al. Prevention and consequences of vitamin D deficiency in pregnant and lactating women and children: a symposium to prioritise vitamin D on the global agenda. J Steroid Biochem Mol Biol 2016;164:156–60.

94. Oberhelman SS, Meekins ME, Fischer PR, Lee BR, Singh RJ, Cha SS, et al. Maternal vitamin D supplementation to improve the vitamin D status of breast-fed infants: a randomized controlled trial. Mayo Clin Proc 2013;88:1378–87.

95. March KM, Chen NN, Karakochuk CD, Shand AW, Innis SM, von Dadelszen P, et al. Maternal vitamin D₃ supplementation at 50 μg/d protects against low serum 25-hydroxyvitamin D in infants at 8 wk of age: a randomized controlled trial of 3 doses of vitamin D beginning in gestation and continued in lactation. Am J Clin Nutr 2015;102:402–10.

96. Tan ML, Abrams SA, Osborn DA. Vitamin D supplementation for term breastfed infants to prevent vitamin D deficiency and improve bone health. Cochrane Database Syst Rev 2020;12:Cd013046.

97. Chandy DD, Kare J, Singh SN, Agarwal A, Das V, Singh U, et al. Effect of vitamin D supplementation, directly or via breast milk for term infants, on serum 25 hydroxyvitamin D and related biochemistry, and propensity to infection: a randomised placebo-controlled trial. Br J Nutr 2016;116:52–8.

98. Naik P, Faridi MMA, Batra P, Madhu SV. Oral supplementation of parturient mothers with vitamin D and its effect on 25OHD status of exclusively breastfed infants at 6 months of age: a double-blind randomized placebo controlled trial. Breastfeed Med 2017;12:621–8.

99. Roth DE. Maternal postpartum high-dose vitamin D3 supplementation (6400 IU/day) or conventional infant vitamin D3 supplementation (400 IU/day) lead to similar vitamin D status of healthy exclusively/fully breastfeeding infants by 7 months of age. Evid Based Med 2016;21:75.

100. Hollis BW, Wagner CL, Howard CR, Ebeling M, Shary JR, Smith PG, et al. Maternal versus infant vitamin D supplementation during lactation: a randomized controlled trial. Pediatrics 2015;136:625–34.

101. European Food Safety Authority (EFSA). Scientific opinion on the tolerable upper intake level of vitamin D. EFSA panel on dietetic products, nutrition and allergies (NDA) [Internet]. Parma (Italy): EFSA; 2012 [cited 2021 Feb 28]. Available from https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/j.efsa.2012.2813.

102. Taylor SN. ABM Clinical Protocol #29: iron, zinc, and vitamin D supplementation during breastfeeding. Breastfeed Med 2018;13:398–404.

103. Wagner CL, Greer FR. Prevention of rickets and vitamin D deficiency in infants, children, and adolescents. Pediatrics 2008;122:1142–52.

104. American Academy of Pediatrics. Statement of endorsement. Dietary reference intakes for calcium and vitamin D. Pediatrics 2012;130:e1424.

105. Vidailhet M, Mallet E, Bocquet A, Bresson JL, Briend A, Chouraqui JP, et al. Vitamin D: still a topical matter in children and adolescents. A position paper by the Committee on Nutrition of the French Society of Paediatrics. Arch Pediatr 2012;19:316–28.

106. Tanaka K, Kuwabara A, Tsugawa N. Vitamin D in the dietary reference intakes for Japanese (DRIs) 2020. J Nutr Sci Vitaminol (Tokyo) 2020;66:497–501.

107. Yorifuji J, Yorifuji T, Tachibana K, Nagai S, Kawai M, Momoi T, et al. Craniotabes in normal newborns: the earliest sign of subclinical vitamin D deficiency. J Clin Endocrinol Metab 2008;93:1784–8.

108. Abrams SA. Calcium and vitamin d requirements of enterally fed preterm infants. Pediatrics 2013;131:e1676–83.

109. Zung A, Topf-Olivestone C, Shinwell ES, Hofi L, Juster-Reicher A, Flidel-Rimon O. Reassessing vitamin D supplementation in preterm infants: a prospective study and review of the literature. J Pediatr Endocrinol Metab 2020;33:1273–81.

110. Agostoni C, Buonocore G, Carnielli VP, De Curtis M, Darmaun D, Decsi T, et al. Enteral nutrient supply for preterm infants: commentary from the European Society of Paediatric Gastroenterology, Hepatology and Nutrition Committee on Nutrition. J Pediatr Gastroenterol Nutr 2010;50:85–91.

111. Chen W, Yang C, Chen H, Zhang B. Risk factors analysis and prevention of metabolic bone disease of prematurity. Medicine (Baltimore) 2018;97:e12861.

112. Pieltain C, de Halleux V, Senterre T, Rigo J. Prematurity and bone health. World Rev Nutr Diet 2013;106:181–8.

113. Lapillonne A, Bronsky J, Campoy C, Embleton N, Fewtrell M, Fidler Mis N, et al. Feeding the late and moderately preterm infant: a position paper of the European Society for Paediatric Gastroenterology, Hepatology and Nutrition Committee on Nutrition. J Pediatr Gastroenterol Nutr 2019;69:259–70.

114. Natarajan CK, Sankar MJ, Agarwal R, Pratap OT, Jain V, Gupta N, et al. Trial of daily vitamin D supplementation in preterm infants. Pediatrics 2014;133:e628–34.

115. Fort P, Salas AA, Nicola T, Craig CM, Carlo WA, Ambalavanan N. A comparison of 3 vitamin D dosing regimens in extremely preterm infants: a randomized controlled trial. J Pediatr 2016;174:132–8.e1.

116. Tergestina M, Rebekah G, Job V, Simon A, Thomas N. A randomized double-blind controlled trial comparing two regimens of vitamin D supplementation in preterm neonates. J Perinatol 2016;36:763–7.

117. Anderson-Berry A, Thoene M, Wagner J, Lyden E, Jones G, Kaufmann M, et al. Randomized trial of two doses of vitamin D₃ in preterm infants <32 weeks: dose impact on achieving desired serum 25(OH)D₃ in a NICU population. PLoS One 2017;12:e0185950.

118. Bozkurt O, Uras N, Sari FN, Atay FY, Sahin S, Alkan AD, et al. Multi-dose vitamin D supplementation in stable very preterm infants: prospective randomized trial response to three different vitamin D supplementation doses. Early Hum Dev 2017;112:54–9.

119. Abdel-Hady H, Yahia S, Megahed A, Mosbah A, Seif B, Nageh E, et al. Mediators in preterm infants with late-onset sepsis: a randomized controlled trial. J Pediatr Gastroenterol Nutr 2019;68:578–84.

120. Mathur NB, Saini A, Mishra TK. Assessment of adequacy of supplementation of vitamin D in very low birth weight preterm neonates: a randomized controlled trial. J Trop Pediatr 2016;62:429–35.

121. Yang Y, Li Z, Yan G, Jie Q, Rui C. Effect of different doses of vitamin D supplementation on preterm infants - an updated meta-analysis. J Matern Fetal Neonatal Med 2018;31:3065–74.

122. Cho SY, Park HK, Lee HJ. Efficacy and safety of early supplementation with 800 IU of vitamin D in very preterm infants followed by underlying levels of vitamin D at birth. Ital J Pediatr 2017;43:45.

123. Justyna CK. Mineral and nutritional requirements of preterm infant. Semin Fetal Neonatal Med 2020;25:101071.

124. Oktaria V, Graham SM, Triasih R, Soenarto Y, Bines JE, Ponsonby AL, et al. The prevalence and determinants of vitamin D deficiency in Indonesian infants at birth and six months of age. PLoS One 2020;15:e0239603.

125. Meena P, Dabas A, Shah D, Malhotra RK, Madhu SV, Gupta P. Sunlight exposure and vitamin D status in breastfed infants. Indian Pediatr 2017;54:105–11.

126. Karthikeyan G, Monish GK. Going solar is good but caution needed! Indian Pediatr 2017;54:511.

127. Jindal AK, Gupta A, Vinay K, Bishnoi A. Sun exposure in children: balancing the benefits and harms. Indian Derm Online J 2020;11:94–8.

128. Balk SJ. Ultraviolet radiation: a hazard to children and adolescents. Pediatrics 2011;127:e791–817.

129. Paller AS, Hawk JL, Honig P, Giam YC, Hoath S, Mack MC, et al. New insights about infant and toddler skin: implications for sun protection. Pediatrics 2011;128:92–102.

130. Aguilar-Shea AL. Vitamin D, the natural way. Clin Nutr ESPEN 2021;41:10–2.

131. Simon AE, Ahrens KA. Adherence to vitamin D intake guidelines in the United States. Pediatrics 2020;145:e20193574.

132. Bilbao NA. Vitamin D toxicity in young breastfed infants: report of 2 cases. Glob Pediatr Health 2017;4:2333794x17731695.

133. Cantrell L, Hogen E. Vitamin D overdosage in an infant from nonprescription vitamin D drops. Am J Health Syst Pharm 2015;72:1262–3.

134. O'Callaghan KM, Taghivand M, Zuchniak A, Onoyovwi A, Korsiak J, Leung M, et al. Vitamin D in breastfed infants: systematic review of alternatives to daily supplementation. Adv Nutr 2020;11:144–59.

135. World Health Organization. Vitamin D supplementation in infants. Geneva (Switzerland): World Health Organization, 2014.