| Role of miRNA-146a and miRNA-125b in Helicobacter pylori |
Nashwa Mohamed1, Ola Behairy1, Manal EL-Defrawy1, Mona Elsayed2 
, Naglaa Alhusseini3 |
1Pediatrics Department, Faculty of Medicine, Benha University, Al-Qalyubia, Egypt, Benha, Egypt
2Pediatrics Department, Shibin El-Qanater Hospital, Al-Qalyubia, Egypt, Benha, Egypt
3Medical Biochemistry and Molecular Biology Faculty of Medicine, Benha University, Al-Qalyubia, Egypt , Benha, Egypt |
Correspondence:
Mona Elsayed, Email: mona.mahmoud27687@gmail.com
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Received: 21 January 2025 • Revised: 25 February 2025 • Accepted: 18 March 2025 |
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| Abstract |
Background
Helicobacter pylori (H. pylori) infection is a common gastrointestinal pathogen associated with gastritis and peptic ulcers. The early detection of H. pylori–related gastritis is crucial to its effective management, especially in pediatric patients with dyspepsia.
Purpose
This study aimed to assess the expression of miRNA-146a and miRNA-125b as potential indicators of H. pylori–associated gastritis in children.
Methods
This cross-sectional study included 70 H. pylori–positive children and 50 H. pylori–negative controls aged ≤ 18 years with recurrent abdominal pain who underwent upper gastrointestinal endoscopy. The miRNA-146a and miRNA-125b expression levels in gastric biopsies were determined using quantitative reverse transcription polymerase chain reaction.
Results
H. pylori–positive children had significantly higher levels of miRNA-146a than H. pylori–negative children across all endoscopic findings (range, 2.2–2.7 vs. 1.07–1.2; p<0.001) and histologic grades of gastritis (range, 2.0–2.4 vs. 1.2–1.3; p<0.001). Conversely, the H. pylori–positive group showed consistently lower miRNA-125b levels across all parameters (range, 0.3–0.8 vs. 0.4–0.9; p<0.001). Both miRNAs differentiated H. pylori status with an area under the curve of >0.95.
Conclusion
The altered expression of miRNA-146a and miRNA-125b in gastric biopsies of H. pylori–positive children suggests their potential role as molecular markers of H. pylori–associated gastritis.
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| Key Words:
Gastritis, Helicobacter pylori, MiRNA-146a, MiRNA-125b |
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