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Original Article
A Study of Receptor Expression in Leukocytes by Quantitative Fluorescence Measurement
Kyung Hyo Kim, Ju Young Seoh
Clin Exp Pediatr. 2002;45(5):637-645.   Published online May 15, 2002
Purpose : It is well known that the receptors for Fcγ moiety of IgG(FcγR) are instrumental in antibody-mediated clearance of microorganisms by granulocytes and monocytes, and immune regulation in lymphocytes. Furthermore, complement receptor-3(CR3) is also important in neutrophilic adhesion, diapedesis, and phagocytic functions. In an attempt to examine their roles in neonates, we compared the expressions of FcγRs and CR3...
Antibody Levels and Opsonophagocytic Function to S. pneumoniae 19F Capsular Polysaccharide
Kyung Hyo Kim, Ju Young Seoh, Moon Kook Park
Clin Exp Pediatr. 2001;44(4):405-412.   Published online April 15, 2001
Purpose : The increasing prominence of pneumococcal infections and of antimicrobial resistance has emphasized the need for a effective vaccine to reduce the risk of pneumococcal disease in childhood. To study the antibody response of pneumococcal vaccine, we compared the concentration and opsonophagocytic capacity of antibodies to the capsular polysaccharide of S. pneumoniae serotype 19F in sera from healthy adults...
Functional Differentiation of HL-60 Cells by Dimethylsulfoxide and Phorbol 12-myristate 13-acetate
Hae Soon Kim, Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh
Clin Exp Pediatr. 1999;42(3):355-363.   Published online March 15, 1999
Purpose : During hematopoietic differentiation of HL-60 cells by DMSO and PMA, we demonstrated functional changes of HL-60 cells-phagocytic activity, respiratory burst, antibody-dependent cell-mediated cytotoxicity(ADCC) and opsonophagocytic activity. Methods : HL-60 cells(ATCC CCL-240), cultured in RPMI 1640 and supplemented with 10% FBS, were induced to differentiate by adding 1.0% DMSO or 16nM PMA. On the 4th and 7th day after...
Immunologic Changes of HL-60 Cells by Differentiation Inducing Agents I : Phenotypic Differentiation of FcγR and Mac-1
Hye Soon Kim, Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh
Clin Exp Pediatr. 1998;41(10):1342-1350.   Published online October 15, 1998
Purpose : Fc receptors and Mac-1 play an important role in the protective response of granulocytes and monocytes against microbial infection. FcγRⅠ, FcγRⅡ, FcγRⅢ as well as CD11b/ CD18 have never been measured in a quantitative way during hemopoiesis. Thus we quantified the expression of FcγRⅠ, FcγRⅡ, FcγRⅢ, and CD11b/CD18 during hematopoietic differentiation using HL-60 cells, which was induced to...
Phenotypic and Functional Differentiation of Promyelocytic Cell Line HL-60 by N-N-dimethylformamide
Kyung Hyo Kim, Ju Young Seoh
Clin Exp Pediatr. 1998;41(4):481-488.   Published online April 15, 1998
Purpose : HL-60 is a promyelocytic cell line. Fcγ receptors and complement receptor 3(CR3) play important role in the protective response of granulocytes and monocytes against microbial infection. We quantified the expression of FcγI, FcγII, FcγIII, and CD11b/CD18 during differentiation using HL-60 cells by N-N-dimethylformamide(DMF). Functional studies, such as phagocytic activity, respiratory burst and ADCC, were also performed. Methods : HL-60 cells were induced to...
The Expression of Fcγ Receptors in Cord and Adult Blood Granulocytes
Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh, Hae-Kyung Park
Clin Exp Pediatr. 1997;40(6):794-800.   Published online June 15, 1997
Purpose : The immature neonatal immune system is thought to result in increased risk of infection. Receptors for the Fc moiety of IgG(FcγR) are important in antibody-mediated clearance of microbes by granulocytes and monocytes/macrophages. As an approach to understand their role in neonatal life, we compared the constitutive expression of the three Fc receptors-FcγRI(CD64), Fcγ RII(CD32) and FcγRIII(CD16)-of neonatal granulocytes with those of adult...