Search

  • HOME
  • Search
Original Article
Functional Differentiation of HL-60 Cells by Dimethylsulfoxide and Phorbol 12-myristate 13-acetate
Hae Soon Kim, Kyung Hyo Kim, Gyoung Hee Kim, Ju Young Seoh
Clin Exp Pediatr. 1999;42(3):355-363.   Published online March 15, 1999
Purpose : During hematopoietic differentiation of HL-60 cells by DMSO and PMA, we demonstrated functional changes of HL-60 cells-phagocytic activity, respiratory burst, antibody-dependent cell-mediated cytotoxicity(ADCC) and opsonophagocytic activity. Methods : HL-60 cells(ATCC CCL-240), cultured in RPMI 1640 and supplemented with 10% FBS, were induced to differentiate by adding 1.0% DMSO or 16nM PMA. On the 4th and 7th day after...