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Original Article
In Vitro Immunization-IV: Antibody Response of Mouse Splenocytes Grown in a Mixture of Conditioned Media, Thymocytes, and Bone Marrow Cells
Dong Soo Kim, Geun Woong Noh
Clin Exp Pediatr. 1992;35(7):902-908.   Published online July 15, 1992
We have previously reported several methods of in vitro immunization using different conditioned media, and the highest antibody titer was observed when a mixture of three conditioned media[PHA-stimulated adherent splenocytes conditioned medium (ASM-P), ASM-p-stimulated non-adherent splenocyte conditioned medium (NASM-A), ASM-P-stimulated thymocyte conditioned medium (TM-A)] was used. In this experiment, thymocytes and/or bone marrow cells was added instead of above conditioned media...
The Effec of Conditiond Media Cultued from the Thymocytes and the Splenocytes on the Regulation of Antibody Isotypes; In Vitro Immunization-III
Dong Soo Kim, Geun Woong Noh, Soon Hwan Oh
Clin Exp Pediatr. 1992;35(3):306-314.   Published online March 15, 1992
We had developed a method of in-vitro immunization to increase antibody titer using several cnditioned media which were developed from mouse splenocyts and thymocytes. Using fetal calf serum, the splenocytes of the mouse were divided into two types; adherent cells and non-adherent cells. We used PHA to stimulate the adherent cells to produce PHA-stimulated adherent cell conditioned medium (ACSM-P). Non-adherent...