| Production, Characterization, and Clinical Application of Perchloric Acid Extract of M. bovis BCG (BCG I) |
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Kyung Hyo Kim1, Dong Soo Kim2 |
1Department of Pediatrics, College of Medicine, Ewha Woman`s University, Seoul, Korea
2Department of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea |
| Perchloric Acid 분리 BCG 항원의 특성과 임상적 의의 (BCG-I) |
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김경효1, 김동수2 |
1이화여자대학교 의과대학 소아과학교실
2연세대학교 의과대학 소아과학교실 |
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| Abstract |
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Tuberculosis has been an important world-wide infectious disease. The prevalence of radiologically active tuberculosis was 1.8% of population in Korea. Probably the most important aspect of the pathogenesis of tuberculosis is the nature and specificity of the response of the immune system to the antigens of tubercle bacilli. Preparation of purified antigens from Mycobacteria with specific antigenic deterninants would improve immunological testing for the diagosis of tuberculosis.
The antigen was prepared by extraction of M. bovis BCG with perchloric acid. The antigen prepared by perchloric acid was found to be a major band of 65 kD by sodium dodecy1 sulphate-polyacrylamide gel electrophoresis. Ten sera from patients with tuberculosis who showed positive reactions to PPD by ELISA, did not react with perchloric acid extract antigen. But, the antigen elicited a delayed cutaneous reaction in BCG vaccinated children.
The results suggest that the perchloric acid extract antigen of M. bovis BCG might be related with cell-mediated immune reactions and will be used as a skin test agent for testing the efficacy of BCG vaccination. |
| Key Words:
M. bovis BCG, Perchloric acid soluble antigen |
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