| Production and Characterization of Monoclonal Antibodies to Perchloric Acid Extract Antigen of M. bovis BCG(BCG Ⅱ) |
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Kyung Hyo Kim1, Dong Soo Kim2, Jeon Soo Shin3 |
1Department of Pediatrics, College of Medicine, Ewha Woman`s University, Seoul, Korea
2Department of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea
3Department of Microbiology, College of Medicine, Yonsei University, Seoul, Korea |
| Perchloric Acid 분리 BCG항원에 대한 단세포군 항체의 제조 및 분석(BCG-Ⅱ) |
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김경효1, 김동수2, 신진수3 |
1이화여자대학교 의과대학 소아과학교실
2연세대학교 의과대학 소아과학교실
3연세대학교 의과대학 미생물학교실 |
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| Abstract |
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Mycobacteria causes disease which occur over the world. The development of reliable serological teset for the diagnosis of tuberculosis in humans has been complicated by the large degree of cross reactivitiy between antigens of Mycobacteria species can be used as a tool for serodiagnosis of tuberculosis. Fourteen monoclonal antibodies to perchloric acid extract antigen of M. bovis BCG were produced. Immunoglobulin isotypes of monoclonal antibodies were all immunoglobulin M. In the autoradiography and colum chromatography, monoclonal antibody detected antigen of 65 kD from M. bovis BCG. In the immunofluorescence assay, the monoclonal antibody showed a positive reaction with intact BCG bacilli and M. tuberculosis in the pulmonary tissue of tuberculous patinet. |
| Key Words:
M. bovis BCG, Monoclonal antibodies |
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