| The Correlation between CD34+ Cell and Nucleated RBC Counts in Human Cord Blood |
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Seong Man Lee, Kyu Chan Kim, Sang Woo Kim |
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Department of Pediatrics, Inje University Paik Hospital, Seoul, Korea |
| 제대혈의 유핵적혈구수와 CD34+ 세포 상관관계에 관한 연구 |
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이성만, 김규찬, 김상우 |
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인제대학교 의과대학 서울백병원 소아과학교실 |
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| Abstract |
Purpose
: Human umbilical cord blood as a potential source of hematopoietic stem cell for
stem cell transplantation in children has recently been advocated. Clinical application of cord
blood transplantation, however, requires adequate blood volume and number of stem cells.
Currently, the number of stem cells in the cord blood is usually measured by flowcytometry,
which requires strict quality control and high costs. Here, we postulate that the number of
nucleated red blood cells(NRBC) which are relatively immature erythroid cells may correlate well
with that of CD34-expressing(CD34+) cells which are considered lymphohematopoietic precursors.
If so, CD34+ cell-rich cord blood can be selected and such cells enumerated by a simple and
cost-effective blood smears.
Methods
: Correlation between CD34+ cell and nucleated RBC in human cord blood were
checked. Sixty cord blood specimens(30 specimens for group A with NRBC>500/μl and 30
specimens for group B with NRBC<500/μl) from healthy newborns were collected in EDTA
bottles and transported to our laboratory at room temperature. CD34+ cells were counted by
flowcytometry(FACScanTM : Becton Dickinson) within 72 hours after collection, and NRBC by
light microscopy.
The results were as following.
Results
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1. The proportion of CD34+ cells in umbilical cord blood was, in the average, 0.85%(±1.1) of
mononuclear cells.
2. There was a significant difference between the means of CD34+ cell counts in group
A(NRBC＜500/ul; 30±48(SD)) and in group B(NRBC＞500/ul; 55±61(SD))(p＜0.005).
3. Regression relationship among CD34+ cell, WBC, and NRBC counts was as following.
ln(CD34+ cell) = -12.21 + 1.46×ln(WBC) + 0.25×ln(NRBC) γ2=30.07 p=0.0001
4. There was a significant correlation between CD34+ cell counts and NRBC counts
(γ=0.4334, p＜0.005)
Conclusion
: Our results suggest that there is a significant correlation between CD34+ cell
counts and NRBC counts, and that CD34+ cell-rich cord blood specimens can be selected by a
simple NRBC counting. |
| Key Words:
CD34+ cell, nucleated RBC, umbilical cord blood stem cell transplantation |
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