| Effect of Meconium Components on Haemophilus influenzae Growth by Surfactan |
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Min Hee Kim, Sang In Chung |
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Department of Pediatrics, Konkuk University, College of Medicine, Seoul, Korea |
| Surfactant의 Haemophilus influenzae 증식억제 효과에 태변이 미치는 영향 |
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김민희, 정상인 |
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건국대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: Meconium is a complex mixture with a potential variable composition, it is
difficulty to determine which components of meconium are responsible for the surfactant inhibition. We previous reported that Surfactant-TA and Exosurf almost completely inhibited the growth of Haemophilus influenzae at the concentration of 100% without apparent difference in the efficiency.In this study, We have determined the effect of Surfactant-TA on H. influenzae growth by meconium and futher investigated if this effect is caused by methanol or chloroform soluble meconium component.
Methods
: Approximately 20gm of meconium was collected from 5 healthy full term
newborn infants.That was suspended in distilled water at a concentration of 25mg/ml
and filtered by Whatmann filter paper No.3. The filtrate was pooled, lyophilized and
stored at -15℃. Filtered meconium was extracted with chloroform-methanol(2:1,v/v).
Exponential growing H. Influenzae(2.7×107cells in 0.1ml of PBS) were mixed with 0.2ml of Surfactant-TA(30mg/ml) at serial dilutions ranging from 0.1% to 100%. Mixed
solution of bacteria, Surfactant-TA, and meconium(0.1ml) or it's subfractions were
incubated at 37℃ for 90 min. After incubation, the mixtures was serially diluted with PBS. Each diluted mixture were cultivated on chocolate agar plate. Volumes of
inoculation were 0.1ml of mixture. Number of viable bacteria were estimated by colony
counting after 18hours culture. For transmission electronmicroscopic finding, mixture of bacteria 100% surfactant-TA were proceeded to prepare.
Results
: 1) Surfactant-TA almost completely inhibited the growth of H. influenzae at the concentration of 100%.
2) The meconium, methanol and chloroform meconium all did not affected the H.
influenzae growth inhibition effect of Surfactant-TA.
3) Surfactant destroyed H. influenzae with disruption of cell membranes and leakage
of intracellular organelles.
Conclusion
: The meconium and it's subfraction all did not affected the H. influenzae growth inhibition effect of Surfactant-TA. So, We speculated that using
Surfactant-TA in meconium aspiration pneumonia will be beneficial to reducing severity of respiratory symptom. But more extensive controlled in vivo test need before surfactant therapy can be considered as a treatment modality for like this condition. |
| Key Words:
Surfactant-TA, Meconium, H. influenza |
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