| Production of Interleukin-1α and Tumor Necrosis Factor-α by Human Cord and Adult Blood Mononuclear Cells Stimulated by TSST-1 and LPS |
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Eun Ae Park, Kyung Hyo Kim, Gyoung Hee Kim |
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Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea |
| 제대혈 및 성인 말초혈 단핵세포에서의 TSST-1과 LPS자극에 의한 IL-1α와 TNF-α의 분비에 관한 비교 연구 |
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박은애, 김경효, 김경희 |
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이화여자대학교 의과대학 소아과학교실 |
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| Abstract |
Purpose
: Immature immunological defens mechanism in the neonate may contribute to the high susceptibility to overwhelming sepsis. S. aureus TSST-1 and E. coli LPS known as one of the important pathogens of septic shock or toxic shock induce massive release of various cytokines, such as tumor necrosis factor-alpha(TNF-α) and interleukin-1α(IL-1α) produced from peripheral blood mononuclear cells(PBMC). In contrast, limited information has been provided so far concerning the capacity of cytokine production from neonatal immune cells.
Methods
: This study was conducted to compare the secretion of proinflammatory cytokines, such as IL-lα and TNF-α from cord blood PBMC to those from adult blood PBMC stimulated by S. aureus TSST-1 and E. coli LPS.
Results
: 1) IL 1-α was secreted in a time-dependent manner from cord & adult blood PBMC stimulated with several cytokine inducers, and LPS stimulated adult & cord blood PBMC secreted IL 1-α in a dose-dependent manner.
2) TNF-α secretion from cord blood PBMC stimulated with LPS and IFN-γ significantly decreased in a time dependent manner, but not from adult PBMC. And secretion of TNF-α from cord blood PBMC reached the highest level 24 hours after stimulated with LPS or IFN-γ. The secretion of TNF-α from adult blood PBMC showed similar pattern to those from cord blood PBMC, but higher than cord blood PBMC.
3) IL-1α & TNF-α secretion from cord & adult blood PBMC stimulated with TSST-1 had no significant difference except in TNF-α secretion by TSST-1 at 96 hours.
4) The secretion of IL-1α from adult PBMC stimulated with LPS showed higher and longer than that from cord blood PBMC.
5) IL-1α & TNF-α secretion from cord & adult blood PBMC stimulated with IFN-γ had no significant difference.
Conclusion
: In summary, proinflammatory cytokines such as IL-1α, TNF-α in cord blood PBMC were secreted in a time dependent manner, but the amounts of IL-1α and TNF-α secretion were lesser than those of adult blood PBMC, especially stimulated by LPS. These results suggest that increased susceptibility to infection in neonatal period may be partially from a functional immaturity of cord blood mononuclear cells. |
| Key Words:
Cord blood PBMC, TSST-1, LPS, TNF-α, IL-1α |
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