| Effect of Oxygen Radicals on Cultured Cerebral Neurons of Neonatal Mouse |
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Yeon-kyun Oh1, Seung-taek Park2 |
1Department of Pediatrics, Wonkwang University School of Medicine, Iksan, Korea
2Department of Anatomy, Wonkwang University School of Medicine, Iksan, Korea |
| 신생쥐의 배양 대뇌신경세포에 대한 산소자유기의 영향 |
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오연균1, 박승택2 |
1원광대학교 의과대학 해부학교실
2원광대학교 의과대학 해부학교실 |
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| Abstract |
Purpose
: In order to elucidate the neurotoxic mechanism of oxygen radicals which
are pathological factor of ischemia, we evaluated the oxidant-induced neurotoxicity and
the neuroprotective effect of antioxidant on cultured cerebral neurons derived from
neonatal mouse.
Methods
: Neurotoxic effect was investigated after cultured mouse neuronal cells were
exposed to oxygen radicals which were generated enzymatically by reaction of xanthine
oxidase(XO) and hypoxanthine(HX). And also the neuroprotective effect of antioxidant
was assessed with catalase. Both effects determined by cell viability were assessesd by
MTT assay and neurofilament enzymeimmuno assay(EIA). In order to see the histologic
change microscopic exam also done on the cerebral neuronal cells.
Results
:
1) Oxygen radicals were toxic on cultured mouse cerebral neurons in dose- and
time-dependent manner.
2) The value of lethal concentration50(LC50) of oxygen radicals was estimated at a
concentration of 25mU/ml xanthine oxidase(XO) and 0.2mM hypoxanthine(HX) in these
culture.
3) Catalase was effective in blocking the neurotoxicity induced by oxygen radicals at a
concentration of 50ug/ml.
4) Oxygen radicals induced the decrease of cell number and the loss of neurites in
cultured mouse cerebral neurons.
Conclusion
: It is suggest that oxygen radicals cause the neurotoxicity and the
selective antioxidants such as catalase are very effective in blocking oxidant-mediated
neurotoxicity on cultured cerebral neurons of neonatal mouse. |
| Key Words:
Oxygen radicals, Catalase, Cerebral neurons |
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