| The Espression of Fcγ Receptors and Mac-1 in Cord and Adult Blood Monocytes and Lymphocytes and Production of Interleukin-8 by Cord and Adult Blood Mononeuclear Cells Stimulated by Lipopolysacchride |
|
Kyung Hyo Kim, Gyoung Hee Kim |
|
Department of Pediatrics College of Medicine, Ewha Woman`s University, Seoul, Korea |
| 제대혈 단핵세포의 Fcγ 수용체와 Mac-1(CR3) 표현 및 Lipopolysaccharide에 의한 Interleukin-8 분비에 관한 연구 |
|
김경효, 김경희 |
|
이화여자대학교 의과대학 소아과학교실, 의과학 연구소 면역학부 |
|
|
|
|
| Abstract |
Purpose
: The immature neonatal immune system is thought to result in increased
risk of infection. Receptors for the Fcmoiety of IgG(FcγR) are important in antibodymediated
clearance of microbes by monocytes/macrophages and immune regulation in
lymphocytes. As an approach to understanding their role in neonatal life, we have compared
the constitutive expression of the three Fc receptors-FcγRI(CD64), FcγRII(CD32)
and FcγRIII(CD16)-by neonatal and adult blood monocytes and lymphocytes. Cells of
monocyte lineage produce substances chemotactic for neutrophils, including interleukin-8
(IL-8). Neonatal monocytes have diminished function compared with adult cells. This
study was designed as an in vitro evaluation of the hypothesis that LPS would induce
IL-8 from monocytes and that neonatal production of this molecule would be impaired.
Methods
: Using quantitative immunofluorescence by flow cytometry, we have compared
the constitutive expression of the three Fc receptors-FcγRI(CD64), FcγRII (CD32)
and FcγRIII(CD16)- and Mac-1(CD11b/CD18) by neonatal and adult blood monocytes and
lymphocytes. The levels of IL-8 released from mononuclear cells after LPS stimulation
from adult and cord blood were checked with sandwich ELISA method and compared.
Results
: The proportions of FcγRI, FcγRII, and Mac-1 positive monocytes were
significanly low in cord blood compared to those of adult. However, in lymphocytes, the
expression of those molecules were not different between cord blood and adult. Adult
mononuclear cell supernatants contained significantly more IL-8 than did neonatal supernatants
in response to LPS.
Conclusion
: There were differences in the expression of Fcγ receptors on monocytes
between cord blood and adult peripheral blood and the effects of LPS on the release of
IL-8 from mononuclear cells were different in adult and cord. This results suggest that
the differences in Fcγ receptor expression may be contributed to the differences in
functional activity and decreased production of IL-8 may contribute to the neonate' s poor
host response to infection. |
| Key Words:
Fcγ receptor, Mac-1, IL-8, Cord blood |
|
PDF Links
Download Citation
