Journal of the Korean Pediatric Society 1998;41(4):472-480.
Published online April 15, 1998.
Preparation and in vitro Physical Activities of Crude Natural Surfactant and Artificial Pulmonary Surfactant Containing Synthetic Peptide and Phospholipid Mixtures
Joo Hyun Kang1, Song Yub Shin1, Cheol-Young Maeng2, Kil Lyong Kim2, Chong-Woo Bae2, Kyung-Soo Hahm2
1Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST
2Department of Pediatrics, College of Medicine, Kyunghee University, Seoul, Korea
천연 폐 계면활성제 및 합성펩타이드와 인지질 혼합물을 이용한 폐 계면활성제의 제조와 표면활성의 물리적 특성에 관한 연구
강주현1, 신송엽1, 맹철영2, 김길룡2, 배종우2, 함경수2
1생명공학연구소 펩타이드공학 Research Unit
2생명공학연구소 펩타이드공학 Research Unit
Kyung-Soo Hahm, Email: 1
: In this study, natural pulmonary surfactant was extracted from bovine lung lavage and its surface activity was determined. To investigate the usefulness of synthetic peptides reconstituted with phospholipid as artificial surfactant, truncated peptides from surfactant protein (SP)-B were synthesized and restored the surface tension lowering activities when appropriately recombined with phospholipid.
: Crude natural surfactant(CNS) was isolated from lung lavage by centrifugation and organic solvent for the extraction of pulmonary surfactant was selected to satisfy the in vitro physical properties. Two truncated peptides derived from C-terminal end of bovine SP-B hydrophobic protein were selected and synthesized. To prepare artificial surfactant, synthetic peptides was added to the phospholipid mixture. The various surfactant mixtures were assayed for in vitro physical activity with the Wilhemly plate method and were determined by surface spreading rate, surface adsorption rate and surface tension-area diagram.
: CNS-chloroform methanol(CM) displayed efficient surface activity compared with clinically used SurfactenⓇ but CNS-BuOH did not. The artificial surfactants containing phospholipid mixture and synthetic peptide were analyzed for their surface activities and displayed significant surfactant properties.
: 1-Butanol or CM(3:1) was used as an extraction solvent for CNS. CNS-CM showed more efficient surface activity than CNS-BuOH. Two synthetic peptides composing artificial pulmonary surfactant were designed and mixing ratio of peptide and phospholipid was established. Artificial surfactant dispalyed weaker surface activity than natural surfactant but significant surfactant activity.
Key Words: Surfactant protein(SP)-B, Surface tension, Synthetic peptide, Respiratory distress syndrome, Wilhemly plate method

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