| Effects of Hyperthermia on Neuronal Nitric Oxide Synthase Expression after Cerebral Ischemia in Gerbils |
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Doo Kwun Kim1, Duk Joon Suh2 |
1Department of pediatrics, Dong-Guk University, College of Medicine, Kyongju, Korea
2Department of Physiology, Dong-A University, College of Medicine, Pusan, Korea |
| 모래쥐에서 뇌허혈 시 Neuronal Nitric Oxide Synthase 유전자 발현에 미치는 고체온증의 효과 |
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김두권1, 서덕준2 |
1동국대학교 의과대학 소아과학교실
2동아대학교 의과대학 생리학교실 |
Correspondence:
Doo Kwun Kim, Email: 1
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| Abstract |
Purpose
: This study was aimed to elucidate the effect of hyperthermia on neuronal nitric oxide synthase(nNOS) expression in both cerebral hemispheres after left common carotid artery occlusion in gerbils.
Methods
: Using Mongolian gerbils, cerebral ischemia was produced by occluding carotid artery for 1-4 hours. Rectal temperature was maintained at 36℃ for normothermia and 40℃ for hyperthermia by heating pad. Western blot and RT-PCR was used to examine the nNOS and the mRNA expression. Neuronal damages were observed by histological study.
Results
: After cerebral ischemia, mRNA of nNOS was expressed more abundantly in ischemic hemisphere than control in both normothermia and hyperthermia. Hyperthermia reduced nNOS protein expression markedly. In pathological study, neurons of hippocampal region were degenerated by ischemia. Hyperthermia by itself induced neuronal degeneration in both control and ischemic region. In immunohistochemistry of brain, there was no significant difference of nNOS expression between normothermia and hyperthermia.
Conclusion
: These findings suggest that increase in body temperature might enhance nNOS mRNA expression but reduce nNOS protein, and that hyperthermia aggravates neuronal damage by ischemia, independent of nNOS gene expression. |
| Key Words:
Gerbil, Hyperthermia, Neuronal Nitric Oxide Synthase, RT-PCR, Western blot |
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