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Effect of Hypoxia-ischemia on c-fos Expression in the Neonatal Rat Brain

Journal of the Korean Pediatric Society 2000;43(3):386-394.
Published online March 15, 2000.
Effect of Hypoxia-ischemia on c-fos Expression in the Neonatal Rat Brain
Wang Bock Lee, Sun Hak Kwon, Heng Mi Kim
Department of Pediatrics, College of Medicine, Kyungpook National University, Taegu, Korea
저산소성 허혈이 신생쥐 뇌 c-fos의 발현에 미치는 영향
이왕복, 권순학, 김행미
경북대학교 의과대학 소아과학교실
Abstract
Purpose
: Brain damage resulting from a combination of hypoxia and ischemia in the newborn infant remains a major cause of perinatal death, cerebral palsy, mental retardation and epilepsy. Metabolic stress, including ischemia, hypoxia and seizures, induces the expression of a variety of stress proteins including nuclear proto-oncogene c-fos. The induction of c-fos can be considered a biomarker of events resulting from ischemia-hypoxia. However, it has been suggested that the mechanism for c-fos activation in the fetal brain is not mature prior to postnatal day 13-21. This study was undertaken to determine the induction of c-fos in neonatal rat brain by hypoxia-ischemia and the regions of brain most vulnerable to hypoxia-ischemia.
Methods
: Ten-day-old postnatal rat pups, subjected to unilateral carotid artery dissection combined with 2-hour hypoxia, were killed at 2 hours and 6 hours after hypoxia-ischemia, and their brains were examined by immunohistochemistry.
Results
: Hypoxia-ischemia induced prominent expression of c-fos in the cingulate cortex and hippocampus in the postnatal rats 2 hours after the insult.
Conclusion
: Hypoxia-ischemia results in increased c-fos expression in 10-day-old rat pups. The results of this experiment also demonstrate that the neonatal rat hippocampus and cortex are the most sensitive brain regions to the induction of c-fos following hypoxia-ischemia.
Key Words: Hypoxia, Ischemia, Newborn rat, c-fos


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