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Apoptosis, P53, bax and Bcl-2 Protein Expressions in Neonatal rat Hippocampus by Kainic Acid-induced Seizure

Journal of the Korean Pediatric Society 2000;43(1):85-96.
Published online January 15, 2000.
Apoptosis, P53, bax and Bcl-2 Protein Expressions in Neonatal rat Hippocampus by Kainic Acid-induced Seizure
Shin Weon Yun, Soo Ahn Chae, Eung sang Choi, Byoung Hoon Yoo
Department of P ediatrics, College of M edicine, Chung-Ang University, Seoul, Korea
신생 쥐 해마에서 Kainic Acid 유발 경련으로 인한 세포자멸사(apoptosis)와 P53, Bax, Bcl-2 단백 발현에 관한 연구
윤신원, 채수안, 최응상, 유병훈
중앙대학교 의과대학 소아과학교실
Abstract
Purpose
: Apoptosis is a process of active cell death which has been suggested to be part of hippocampal cell loss caused by kainic acid(KA). Immature rats showed higher susceptibility and mortality to KA but did not develop recurrent seizure, long term behavioral or neuropathologic changes. We investigated whether this was due to age-dependent resistance, and elucidated the molecular mechanics which mediate P53-induced apoptosis, identifying bax and bcl-2 as P53 protein expressions that serve as a paradigm on how the balance of bcl-2 to bax is differentially altered by apoptotic stimuli.
Methods
: Sprague-Dawley rats were classified into postnatal age(P) 10, 15, 20 and 30 days and given specific doses of i.p. KA(P10; 3mg/kg, P15; 4mg/kg, P20; 8mg/kg, P30; 10mg/kg). Only rats that achieved continuous clonic seizure were selected and decapitated at 24, 48, and 72h(n=15 each age). We analysed P53, bax and bcl-2 protein expressions by immunohistochemistry as well as apoptosis by TUNEL in each group.
Results
: KA-induced hippocampal cell death first appeared in P20. Remarkable expressions of apoptosis, P53 and bax, while bcl-2 proteins were suppressed were observed at 48 hours following KA in P20 and the most prominently affected areas were hippocampal CA1 and CA3 neurons. Similar results were obtained in P30.
Conclusion
: Induction of P53, a growth regulatory gene which has been implicated in apoptosis and the changes in bcl-2 to bax ratio may be important to KA mediated excitotoxicity of specific regions during the critical period of developing rat brain.
Key Words: Kainic acid(KA), Hippocampus, Immature rat, Apoptosis, P53, Bax, Bcl-2 protein


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