Korean Journal of Pediatrics 2005;48(8):877-880.
Published online August 15, 2005.
Clinical Findings of Genotypes in Korean Patients with Glycogen Storage Disease Type Ia
Jae Sung Ko1, Hye Ran Yang1, Jong Won Kim2, Jeong Kee Seo1
1Department of Pediatrics, Seoul National University College of Medicine, Seoul, Korea
2Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
한국인 당원병 제 Ia형에서 유전형의 임상 양상
고재성1, 양혜란1, 김종원2, 서정기1
1서울대학교 의과대학 소아과학교실
2성균관대학교 의과대학 삼성서울병원 진단의학과
Correspondence: 
Jeong Kee Seo, Email: jkseo@snu.ac.kr
Abstract
Purpose
: Glycogen storage disease type Ia(GSD Ia) is an autosomal recessive disorder caused by the deficiency of glucose-6-phosphatase(G6Pase). The aim of the study was to investigate the spectrum of G6Pase gene mutations and relationship between genotype and clinical findings in Korean patients with GSD Ia.
Methods
: Genomic DNA was extracted from peripheral leukocytes of 20 patients with GSD Ia. The five exons of G6Pase gene were amplified and PCR products were directly sequenced. The frequency of short stature, hypoglycemia, hypercholesterolemia, hyperuricemia, hypercalciuria, nephrocalcinosis and hepatic adenoma was compared between 727G>T homozygotes and 727G>T compound heterozygotes.
Results
: A total of 5 different mutations were identified. The most common mutation was the 727G>T with an allele frequency of 80%. All patients were either homozygous(12/20) or heterozygous(8/20) for the 727G>T mutation. G122D was found in 3 patients, P178A in 1, G222R in 2, and S339R in 2. There was no difference in the frequency of short stature, hypoglycemia, hypercholesterolemia, hyperuricemia, nephrocalcinosis, and hepatic adenoma between 727G>T homozygotes and heterozygotes.
Conclusion
: Diagnosis of GSD Ia can be based on clinical and biochemical abnormalities combined with mutation analysis instead of enzymatic diagnosis that requires liver biopsy. Homozygosity for the 727G>T does not seem to alter the disease phenotype as compared with the heterozygous state.
Key Words: Glycogen storage disease type Ia , Glucose-6-phosphatase , Mutation , Clinical finding , Korean


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