| Pneumococcal Surface Protein A of Streptococcus pneumoniae Isolates from Koreans |
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Kyung Hyo Kim |
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Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea |
| 한국인에서 Streptococcus pneumoniae 분리주의 폐구균 표면 단백 A |
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김경효 |
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이화여자대학교 의과대학 소아과학교실 |
Correspondence:
Kyung Hyo Kim, Email: kaykim@ewha.ac.kr
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| Abstract |
Purpose
: Pneumococcal protein vaccine based on pneumococcal surface protein A (PspA) is in development with the potential to offer a broad range of protection against different strains. PspA elicits protection in mice against fatal sepsis as well as carriage and lung infection. This study was performed to investigate the frequency of PspA families among Streptococcus pneumoniae recovered from Korean children and adults.
Methods
: A total of 89 pneumococcal isolates was included in the study. They were capsule serotyped by the slide agglutination assay with commercial antisera. PspA families were determined with polymerase chain reaction using the pair of primers for family 1 and family 2.
Results
: Seventeen pneumococcal serotypes were found in a total of 89 isolates. PspA typing was able to ascertain 79 of the 89 isolates (88.8 percent). Among these, 20 (22.5 percent) isolates were family 1 PspA, 59 (66.3 percent) were family 2. Moreover, because 9 (10.1 percent) isolates were of positive reactions for both, families 1 and 2 primers, the potential coverage of PspA vaccine was 98.9 percent. PspA families were not associated with age group, source of isolates, or penicillin susceptibility. However, the relative distribution of family 1 isolates to family 2 isolates was significantly different over capsular serotypes.
Conclusion
: The finding that 98.9 percent of Korean isolates belonging to PspA families 1 and 2 support the hypothesis that a human PspA vaccine covering a few PspA families could be broadly effective. The monitoring of the PspA families derived from large population-based isolates will be necessary in the context of vaccine development. |
| Key Words:
Streptococcus pneumoniae , Pneumococcal surface protein A |
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