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A study of serotyping of Streptococcus pneumoniae by multibead assay

Korean Journal of Pediatrics 2007;50(2):151-156.
Published online February 15, 2007.
A study of serotyping of Streptococcus pneumoniae by multibead assay
Ky Young Cho1, Jung Ah Lee1, Sung Eun Cho2, Nam Hee Kim3, Jin A Lee3, Ki Sook Hong2, Hoan Jong Lee3, Kyung Hyo Kim1
1Departments of Pediatrics, College of Medicine Ewha Woman's University
2Departments of Laboratory Medicine, College of Medicine Ewha Woman's University
3Department of Pediatrics, College of Medicine, Seoul National University
다중구슬 분석법에 의한 폐구균 혈청형 결정 연구
조기영1, 이정아1, 조성은2, 김남희3, 이진아3, 홍기숙2, 이환종3, 김경효1
1이화여자대학교 의과대학 소아과학교실
2이화여자대학교 의과대학 진단검사의학교실
3서울대학교 의과대학 소아과학교실
Correspondence: 
Kyung Hyo Kim, Email: kaykim@ewha.ac.kr
Abstract
Purpose
: Streptococcus pneumoniae is a major etiologic agent for pneumonia, meningitis, otitis media, and sepsis among young children. Multi-drug resistant strains have raised great concern worldwide, thus the importance of prevention with vaccines has been emphasized. However, vaccines may force the appearance of pneumococcal infections by nonvaccine serotypes. Thus, distribution of pneumococcal serotypes should be monitored to estimate vaccine efficacy. We used a new and efficient multibead assay in determining pnemococcal serotypes.
Methods
: From January to February 2005, 643 children were recruited from ten day care centers to isolate pneumococci from their oropharynx. Pneumococcal serotyping was performed on 62 pneumococcal isolates from 60 children by multibead assay. This immunoassay required two sets of latex particles coated with pneumococcal polysaccharides and serotype-specific antibodies. Twenty four newly developed monoclonal antibodies specific for common serotypes and a pool of polyclonal rabbit sera for some of the less common serotypes were used.
Results
: The most prevalent pneumococcal serotypes were serotype 6A, 19A, 19F, 23F, and 11A/ D/F which accounted more than 50 precent of all the 62 pneumococcal isolates. We found that multibead assay can be performed very rapidly and objectively.
Conclusion
: This multibead immunoassay was very useful in serotyping clinical isolates of S. pneumoniae because it was simple, reliable and fast.
Key Words: Streptococcus pneumoniae , Serotyping , Immunoassay


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