| Molecular detection of Bartonella henselae DNA from fleas obtained from dogs, Korea |
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Tae Hee Han1, Ju-Young Chung2, Hee Kyung Seong3, Sang Woo Kim2 |
1Departments of Pediatrics, Inje University Sanggye Paik Hospital, Inje University College of Medicin
2Departments of Laboratory Medicine, Inje University Sanggye Paik Hospital, Inje University College o
3Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje Uni |
| 개에서 추출된 벼룩에서 분자생물학적 방법에 의한 Bartonella henselae DNA의 검출 |
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한태희1, 정주영2, 성희경3, 김상우2 |
1인제대학교 의과대학 진단검사의학과교실
2제대학교 의과대학 소아과학교실
3인제대학교 생명과학대학교 생명과학연구소 |
Correspondence:
Ju-Young Chung, Email: pedchung@sanggyepaik.ac.kr
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| Abstract |
Purpose
: The purpose of this study was to investigate the presence of Bartonella henselae DNA, which is known as an etiologic agent of lymphadenitis, in fleas from dogs.
Methods
: The Bartonella henselae infection was investigated in 42 fleas from 22 dogs in Korea. By using seminested PCR targeting pap31 gene, B. henselae DNA was amplified from fleas.
Results
: B. henselae DNA was detected in seven fleas (7 of 42 fleas, 16.7 percent) from four dogs (4 of 22 dogs, 18.2 percent). To confirm these findings, we performed sequencing and identified the seven PCR products. Sequence analysis revealed that six sequences belonged to Huston-1 genogroup and one sequence to Marseille genogroup.
Conclusion
: These results may suggest that dogs could be an important source of B. henselae infection in children in Korea. This is the first report about the detection of B. henselae in fleas from dogs in Korea. |
| Key Words:
Bartonella henselae , Fleas , Dogs , Polymerase chain reaction |
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